C反应蛋白测定两种检测系统间结果的比较

目的 对两种不同检测系统测定C反应蛋白(C-reactive protein,CRP)的结果进行比对和偏倚分析,以评价不同检测系统CRP测定结果的可比性。方法 参照美国临床实验室标准研究所(CLSI)EP9-A2文件要求,收集长海医院急诊患者乙二胺四乙酸二钾(EDTA-K₂)抗凝血50例,同时使用全自动免疫分析仪(Beckman Immage 800)和免疫荧光分析仪(i-CHROMA^TM Reader)检测待测样本中的CRP浓度,评价两种方法检测结果的相关性。结果 使用血浆标本时,两方法相关性良好,回归方程为Y=1.076 5X-3.031 5,R²=0.986。全血测定的回归方程为Y=0.882 6X-1.180 8,R²=0.931 1。对于红细胞压积(HCT)较低的标本(<30.45%)使用纠正公式:全血CRP(纠正后)=全血CRP结果(纠正前)/(1-HCT)。Y(纠正后)=1.006 8X-3.612 2,R²=0.950 9。结论 两种检测系统血浆CRP的测定结果具有可比性,适合临床应用。遇HCT较低的标本时,通过校正公式校正,可提高全血结果的可比性。

Comparison of Different Assay System for C-reactive Protein Detection

Objective To evaluate comparability of two different assay system for detecting CRP.Methods Following the profile of Clinical and Laboratory Standard Institute(CLSI)document EP9-A2, 50 blood samples with anti-coagulant EDTA-2K were collected from emergencypatients at Changhai Hospital.The test result of samples by the i-CHROMA Reader was compared and evaluated with those by Beckman Immage 800.Results The linear regression equation for plasma CRP was:Y=1.076 5X-3.031 5,R²=0.986.The linear regression equation for whole blood CRP was:Y=0.882 6X-1.180 8,R²=0.931 1.For whole blood samples with low HCT(<30.45%).Used correction equation:CRP(after corrected)=CRP(before corrected)/(1-HCT).The regression equation(after corrected)was:Y=1.006 8X-3.612 2,R²=0.950 9.Conclusion CRP concentration detected by i-CHROMA showed good correlation and comparability comparedto laboratory reference system by using plasma samples.Results form whole bloodsamples with low HCT should be corrected to improve comparability.