三种特殊饮用水对小鼠免疫系统的影响

目的　了解碱性离子水、矿溶生态水、活化水对机体免疫系统的影响。方法　根据《保健食品功能学评价程序和检验方法 1 996》 ,选用 1 2 0只 1 7 8～ 2 3 3g健康雄性Balb c小鼠 ,分为 4小组 ,每小组 30只 ,每天分别自由饮用纯净水 (对照组 )、碱性离子水、矿溶生态水、活化水 ,5 0天后进行小鼠脏器体重比值测定、迟发型变态反应实验、腹腔巨噬细胞吞噬鸡红细胞实验、半数溶血值 (HC50 )的测定和抗体生成细胞数的测定(Ⅰ组 )。另选用 1 2 0只 1 7 8～ 2 3 3g健康雄性Balb c小鼠 ,分组同前 ,5 0天后进行ConA诱导的小鼠淋巴细胞转化实验和NK细胞活性测定 (Ⅱ组 ) ,再选用 1 2 0只 1 6 7～ 2 2 0g健康雌性Balb c小鼠 ,分组同前 ,5 0天后进行小鼠碳廓清实验 (Ⅲ组 )。实验数据用stata软件 ,多个实验组和一个对照组间比较 ,进行方差分析。结果　 (1 )三种特殊饮用水与纯净水比较 ,对雌性Balb c小鼠体重增长的影响不显著 ,但饮用碱性离子水和活化水的雄性小鼠的体重增长值均明显低于纯净水 (P <0 0 1和P <0 0 5 ) ;(2 )活化水与纯净水组间比较 ,胸腺体重比值明显增加 (P <0 0 5 ) ;腹腔巨噬细胞鸡红细胞吞噬指数明显增加 (P <0 0 1 ) ;NK细胞活性明显增加(P <0 0 1 )。 (3)其他指标与相应的纯净水比较差?

Impact of extra waters on immunosystem in mice

OBJECTIVE: To study impact of extra waters on immunosystem in mice-alkaline ionized water, mineral-ecology water, activated water, and pure water. METHODS: According to Function Assessment and Experiment for Function Food, 1996, the ratio of spain and body weight, the ratio of thymus and body weight, the delayed type hypersensitivity (DTH), the phagocytosing functions by cock RBC, the plaque forming cell (PFC) and HC50 testing were assessment with 120 male Balb/c mice of 17.8 - 23.3 g (group I), the mice were divided into four group, and drank daily the four kinds of waters respectively until 50 days. The lymph cell transformation by ConA and NK cell activity were assessment with other l20 male Balb/c mice of 17.8 - 23.3 g (group II), the mice were divided into four group and drank daily the four kinds of waters respectively until 50 days. The phagocytosing functions by carbon powder were assessment with other 120 female Balb/c mice of 16.7 - 22.0 g (group III) were divided into four group and drank daily the four kinds of waters respectively until 50 days. The data were statisticed by Stata soft. RESULTS: Other three waters compared with the pure water: (1) Alkaline ionized water and activated water can alleviate the body weight increase of male Balb/c mouse (P < 0.01 & P < 0.05), but alkaline ionized water, mineral-ecology water, activated water don't impact on the female Balb/c mouse body weight (P > 0.05). (2) Activated water can remarkably increase the ratio of thymus and body weight (P < 0.05), and increase the phagocytosing ability by cock RBC (P < 0.01), and increase the NK cell activity (P < 0.01). (3) The three extra waters don't impact on other items for the Balh/c mouce. CONCLUSION: The study must be continued to impact on immunosystem in mice for extra waters.