罗非鱼肉蛋白酶解液的制备及体内外抗氧化活性研究

采用木瓜蛋白酶酶解罗非鱼肉蛋白，通过测定不同剂量罗非鱼肉蛋白酶解液的还原力，1,1-二苯基-2-三硝基苯肼（1,1-diphenyl-2-picrylhydrazyl radical 2,2-diphenyl-1-（2,4,6-trinitrophenyl）hydrazyl，DPPH）自由基、·OH和O2－·清除率及对D-半乳糖致衰老模型小鼠的抗氧化作用，评价罗非鱼肉蛋白酶解液的体内外抗氧化特性。结果表明：罗非鱼肉蛋白酶解液具有较高的还原力，清除DPPH自由基、·OH和O2－·的半数有效质量浓度分别为1.57、1.68 mg/mL和2.76 mg/mL；罗非鱼肉蛋白酶解液能显著提高小鼠血清和肝脏组织中超氧化物歧化酶活力、谷胱甘肽过氧化物酶活力和总抗氧化能力（P＜0.05），同时使血清和肝脏组织中的丙二醛含量显著降低（P＜0.05）。罗非鱼肉蛋白酶解液有较强的体内外抗氧化活性。

Preparation of Tilapia (Oreochromis niloticus) Protein Hydrolysate and Analysis of Its Antioxidant Activity in vitro and in vivo

Tilapia (Oreochromis niloticus) protein hydrolysate was prepared with papain. Its antioxidant activitiesat different doses were evaluated in vitro and in vivo using reducing power, 1,1-diphenyl-2-picrylhydrazyl radical2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl (DPPH), hydroxyl and superoxide anion radical scavenging assaysand D-galactose-induced mouse aging model. The reducing power of the hydrolysate was high. The EC50 valuesin the DPPH radical, hydroxyl and superoxide anion radical scavenging assays were 1.57, 1.68 and 2.76 mg/mL,respectively. The hydrolysate could significantly increase the activities of superoxide dismutase (SOD) andglutathione peroxidase (GSH-Px) and total antioxidative capacity (T-AOC) in the serum and liver of aging mice(P ＜ 0.05). Furthermore, the content of malondialdehyde (MDA) in serum and liver displayed a reducing trend (P ＜ 0.05).In conclusion, the tilapia protein hydrolysate has a good antioxidant effect in vitro and vivo as expected.