| 响应面法优化碱性蛋白酶酶解草鱼蛋白质 |
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| 引用本文： | 石岭,赵利,袁美兰,苏伟,刘华,陈丽丽,温慧芳.响应面法优化碱性蛋白酶酶解草鱼蛋白质[J].食品科学,2014,35(4):26-29. |
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| 作者姓名： | 石岭 赵利 袁美兰 苏伟 刘华 陈丽丽 温慧芳 |
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| 作者单位： | 江西科技师范大学生命科学学院，国家淡水鱼加工技术研发分中心（南昌），江西 南昌 330013 |
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| 基金项目： | 江西省高等学校科技落地计划项目(KJLD12009) |
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| 摘 要： | 用Alcalase碱性蛋白酶对草鱼蛋白质进行酶解，比较酶的添加量、pH值、温度、料液比以及酶解时间对草鱼蛋白水解进程的影响，通过响应面法优化Alcalase碱性蛋白酶酶解反应的工艺条件。结果表明：Alcalase碱性蛋白酶酶解草鱼蛋白质的最优工艺条件为：加酶量1.8%、酶解温度55 ℃、酶解pH 9.0、料液比1∶15、酶解时间180 min，此条件下蛋白质水解度达到23.46%。
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| 关 键 词： | 草鱼蛋白 酶解 响应面 |
| 收稿时间： | 2013-04-25 |
Optimizing Conditions for Alcalase-Catalyzed Hydrolysis of Grass Carp Protein by Response Surface Methodology |
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| SHI Ling,ZHAO Li,YUAN Mei-lan,SU Wei,LIU Hua,CHEN Li-li,WEN Hui-fang.Optimizing Conditions for Alcalase-Catalyzed Hydrolysis of Grass Carp Protein by Response Surface Methodology[J].Food Science,2014,35(4):26-29. |
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| Authors: | SHI Ling ZHAO Li YUAN Mei-lan SU Wei LIU Hua CHEN Li-li WEN Hui-fang |
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| Affiliation: | National R&D Branch Center for Freshwater Fish Processing, College of Life Science,
Jiangxi Science and Technology Normal University, Nanchang 330013, China |
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| Abstract: | This study aimed to optimize the conditions for enzymatic hydrolysis of grass carp proteins. Alcalase was used
to hydrolyze grass carp proteins. The effects of enzyme/substrate ratio, pH value, temperature, material/liquid ratio and
hydrolysis time on the degree of hydrolysis were analyzed. Box-Behnken design (BBD) and response surface methodology
(RSM) were adopted to obtain the optimum hydrolysis conditions on the basis of results of single-factor experiments. The
degree of hydrolysis of 23.46% was obtained under the optimum hydrolysis conditions of 1.8%, 55 ℃, 1:15, 180 min and 9.0
for enzyme/substrate ratio, temperature, material/liquid ratio, hydrolysis time, and pH, respectively. |
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| Keywords: | grass carp protein enzymatic hydrolysis response surface methodology |
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