| 液相色谱-串联质谱联用法测定心脑欣片中甜菜碱含量 |
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| 引用本文： | 赵勇,;王艳,;丁野,;郑金凤,;李文莉.液相色谱-串联质谱联用法测定心脑欣片中甜菜碱含量[J].中国药业,2014(15):39-41. |
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| 作者姓名： | 赵勇 ;王艳 ;丁野 ;郑金凤 ;李文莉 |
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| 作者单位： | [1]湖南省食品药品检验研究院,湖南长沙410001; [2]湖南中医药大学药学院,湖南长沙410208 |
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| 摘 要： | 目的：建立测定心脑欣片中甜菜碱含量的方法。方法采用液相色谱-串联质谱联用（LC - MS / MS）法测定甜菜碱的含量，以电喷雾离子源（ESI）正离子模式将样品离子化，多反应监测（MRM）模式下对甜菜碱（ m / z 118.20→57.92和118.20→59.00）进行测定。色谱柱为 Venusil HILIC 柱（100 mm×2.1 mm，3μm），流动相为乙腈-水（80：20），流速为0.3 mL / min。结果甜菜碱进样质量浓度在4.44-177.6 ng / mL 范围内与峰面积积分值线性关系良好（ r =0.9999），平均回收率为99.45%，RSD 为1.43%（ n =6）。结论该方法简便、可靠、准确，可作为测定心脑欣片中甜菜碱含量的方法。
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| 关 键 词： | 心脑欣片 甜菜碱 液相色谱-串联质谱联用法 |
Determination of Betaine Content in Xinnaoxin Tablet by LC - MS/MS |
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| Affiliation: | Zhao Yong, Wang Yan, Ding Ye, Zheng Jinfeng, Li Wenli(1. Hunan Provincial Institute for Food and Drug Control, Changsha, Hunan, China 410001; 2. Pharmacy College, Hunan University of Chinese Medicine, Changsha, Hunan, China 410208) |
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| Abstract: | Objective To establish the method for determining the betaine contents in Xinnaoxin Tablet. Methods The betaine content in Xinnaoxin Tablet was determined by LC - MS / MS. The sample was ionized by the eletrospray ionization（ESI） positive ion mode and betaine（ m / z 118. 20→57. 92 and 118. 20→59. 00） was detected under the multiple reaction monitor（MRM） mode. The chromatographic column was the Venusil HILIC column（100 mm × 2. 1 mm × 3 μm） . The mobile phase was acetonitrile - water（80 ： 20）, the flow rate was 0. 3 mL / min. Results The mass concentration of betaine sample size in the range of 4. 44 - 177. 6 ng / mL exhibited the good linear relation（ r = 0. 999 9）. The average recovery rate was 99. 45% , RSD 1. 43% （ n = 6）. Conclusion This method is simple, reliable and ac-curate, which can be used to determine the betaine content in Xinnaoxin Tablet. |
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| Keywords: | Xinnaoxin Tablet betaine LC - MS/MS |
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