桦褐孔菌子实体与菌丝体三萜化合物提取及活性比较研究

为探索桦褐孔菌菌丝体与子实体在药用价值上是否存在差别,采用有机溶剂冷浸、超声回流法提取桦褐孔菌菌丝体及子实体的三萜化合物,通过薄层层析和红外光谱分析,确定两种来源的三萜化合物是相同或近似的同系物。四甲基偶氮唑盐比色法(MTT)和杯碟法测定两种来源三萜化合物对癌细胞和细菌的抑制作用,菌丝体三萜化合物对人胃癌细胞株MGC-803的最高抑制率可达到38.27%。结果证实人工培养的桦褐孔菌菌丝体同样具有子实体的药用价值,为人工培养桦褐孔菌菌丝体提供了依据。

Extraction and Bioactivity of Triterpenoids from Fruit Bodies and Mycelia of Inonotus obliquus

In order to explore the difference in medicinal value of cultured mycelia and fruit bodies of Inonotus obliquus,organic solvent cold soaking and ultrasonic reflux were adopted to extract triterpenoids from fruit bodies and mycelia of Inonotus obliquus.The triterpenoids extracted from both sources were determined to be the same or similar homologues by thin layer chromatography(TLC) and Fourier transform infrared(FT-IR) spectral analysis.Both triterpenoid extracts could inhibit the growth of cancer cells and bacteria as determined by tetrazolium salt assay(MTT) and cylinder plate method.The highest inhibitory rate of triterpenoids to human gastric cancer cell line MGC-803 reached up to 38.27%.Therefore cultured mycelia of Inonotus obliquus have the same medicinal value as fruit bodies.