醋酸菌产乙醇脱氢酶发酵条件的研究

该研究对醋酸菌（Acetobacter pomorum）产乙醇脱氢酶（ADH）的发酵培养基的碳源和氮源进行了优化。采用单因素试验和响应面试验考察发酵温度、发酵时间、起始pH及接种量对乙醇脱氢酶比酶活的影响。在单因素试验的基础上，采用响应面试验进行发酵条件优化。结果表明，最佳发酵培养基配方为葡萄糖0.8%，酵母浸膏粉1.2%；最佳发酵工艺为发酵温度35 ℃、发酵时间72 h、起始pH 6.0、接种量6%。在此工艺条件下，ADH的比酶活为7 284 U/mg，与预测值（7 320 U/mg）的相对误差为0.49%。

Optimization of fermentation conditions of alcohol dehydrogenase from Acetobacter pomorum

The carbon and nitrogen sources in fermentation medium for alcohol dehydrogenase (ADH) production by Acetobacter pomorum were optimized in this paper. The effects of fermentation temperature, time, initial pH and inoculum on ADH specific enzyme activity were investigated by single factor experiments and response surface methodology. Based on single factor experiments, fermentation conditions were optimized by response surface experiments. Results showed that the optimum fermentation medium formula was glucose 0.8% and yeast extract 1.2%. The optimum fermentation conditions were temperature 35 ℃, time 72 h, initial pH 6 and inoculum 6%. Under the conditions, the ADH specific enzyme activity was 7 284 U/mg, and the relative error was 0.49% compared with the predicted value (7 320 U/mg).