PD-1、CTLA-4、BLTA在食管鳞状细胞癌患者外周血中的表达及临床意义

目的 检测食管鳞癌患者外周血中抑制性协同刺激因子受体PD-1、CTLA-4、BLTA表达情况,并分析其临床意义。方法 选取2016年6月—2017年4月河北医科大学第四医院胸外科90例食管鳞状细胞癌患者(其中50例患者行手术治疗)和40例健康对照者为研究对象,收集其外周血液标本,采用酶联免疫吸附方法检测血清中可溶性PD-1(sPD-1)、可溶性CTLA-4(sCTLA-4)及可溶性BLTA(sBLTA)的表达水平。结果 食管鳞癌组血清中sPD-1、sCTLA-4及sBLTA水平均明显高于对照组(P＜0.05);食管鳞癌组手术前后血清中sPD-1、sCTLA-4及sBLTA水平均无统计学差异(P＞0.05)。sPD-1和sBLTA的表达水平与临床病理特征无相关性,sCTLA-4的表达水平与TNM分期相关(P＜0.05),与T分期、N分期、肿瘤体积大小、肿瘤部位、组织分化程度、性别、年龄无相关性;血清中sPD-1、sCTLA-4及sBLTA两两间无相关性(P＞0.05)。结论 食管鳞癌患者血清中sCTLA-4较正常人表达升高,且sCTLA-4的表达水平与TNM分期相关,说明血清中sCTLA-4表达水平与病情发展变化有一定相关性。

Expression of PD-1,CTLA-4 and BLTA in the peripheral blood of patients with esophageal squamous cell carcinoma and its clinical implications

Objective The aim of this study was to evaluate the expression of programmed cell death 1(PD-1),cytotoxic T lymphocyte associated antigen-4(CTLA-4)and B and T lymphocyte attenuator(BTLA)in the peripheral blood of patients with esophageal squamous cell carcinoma(ESCC)and their clinical significance was analyzed. Methods A total of 90 patients with esophageal squamous cell carcinoma and 40 healthy controls at the department of Thoracic Surgery of the Fourth Hospital of Hebei Medical University were enrolled and collected their peripherals from June 2016 to April 2017.Blood samples from 50 patients underwent surgery from 90 patients and 40 healthy controls were assayed by enzyme-linked immunosorbent assay for soluble PD-1(sPD-1),soluble CTLA-4(sCTLA-4)and soluble BTLA(sBTLA). Results The serum levels of sPD-1,sCTLA-4 and sBLTA in the esophageal squamous cell carcinoma group were significantly higher than those in the normal control group(P＜0.05);the serum levels of sPD-1,sCTLA-4andsBLTA had no differences between before and after surgery(P＞0.05).There was no correlation between the level of sPD-1 and sBLTA and clinicopathological features.The expression level of CTLA-4 was related to the TNM stage(P＜0.05),but there were no correlation with T stage,lymph node status,tumor size,tumor location,tumor cell differentiation,sex and age.There was also no correlation amongst sPD-1,sCTLA-4 and sBLTA expression. Conclusion The serum level of sCTLA-4 in patients with esophageal squamous cell carcinoma is higher than that in normal individuals,and the expression level of sCTLA-4 is correlated with the TNM stage,suggesting that the expression level of sCTLA-4 in serum has a certain correlation with the development of esophageal squamous cell carcinoma.