HPV E6/E7 mRNA与YAP在宫颈癌中的表达与相关性研究

目的:研究宫颈病变中HPV E6/E7 mRNA与Yes相关蛋白（YAP）的表达情况及临床意义，探讨两者之间的相关性。方法: 应用支链DNA（b-DNA）技术检测36例正常宫颈组织（正常组）、45例宫颈上皮内瘤变(CIN) Ⅱ-Ⅲ级（CIN组）、43例宫颈癌（宫颈癌组）的宫颈脱落细胞标本中HPV E6/E7 mRNA的表达情况，免疫组化方法（IHC）检测上述各组患者宫颈组织石蜡包埋标本中YAP的表达情况。结果:HPV E6/E7 mRNA及YAP阳性表达率在正常组分别为16.67%、11.11%，在CIN组分别为82.22%、84.44%，在宫颈癌组分别为88.37%、90.70%，3组HPV E6/E7 mRNA及YAP阳性表达率均逐渐升高，且差异均具有统计学意义（P<0.05）。YAP在宫颈癌有淋巴结转移组中的表达明显高于无淋巴结转移组（P<0.05），而与宫颈癌的临床分期、组织学分级及组织学类型无关（P>0.05)。HPV E6/E7 mRNA与YAP的表达呈正相关（r=0.383，P<0.05）。结论:HPV E6/E7 mRNA与YAP的检出率随病理级别的升高呈增强趋势，且两者具有相关性。因此，HPV E6/E7 mRNA与YAP的联合检查，可以提高宫颈病变的诊断率并能够预测癌前病变的进展。

HPV E6/E7 mRNA and YAP expression and their correlation in cervical carcinoma

Objective: To investigate the expression and clinical significance of human papillomavirus(HPV) E6/E7 mRNA and Yes-associated protein (YAP) in the cervical lesions, and to explore the correlation between them. Methods: Branched DNA (b-DNA) technology was used to detect the expression of HPV E6/E7 mRNA in 36 cases of normal cervical tissues (normal group), 45 cases of cervical intraepithelial neoplasia(CIN) II-III(CIN group), and 43 cases of cervical carcinoma(cervical carcinoma group). Immunohistochemistry（IHC） method was used to examine the expression of YAP in the paraffin embedded specimens of cervical tissue of the above groups. Results: The positive rates of HPV E6/E7 mRNA and YAP in normal group were 16.67% and 11.11%, 82.22% and 84.44% in CIN group, and 88.37% and 90.70% in cervical carcinoma group. The positive rates of HPV E6/E7 mRNA and YAP were all gradually increased in the three groups, and the difference among the three groups was statistically significant (P<0.05). In cervical carcinoma, the expression of YAP in node-positive cases is significantly higher than node-negative cases (P<0.05), whereas no relations to clinical stage， histological grade and histological types of cervical carcinoma were found (P>0.05). The expression of HPV E6/E7 mRNA and YAP was positively correlated(r=0.383，P<0.05). Conclusion: The positive rate of HPV E6/E7 mRNA and YAP increased with the increase of pathological grade, and the expression of HPV E6/E7 mRNA and YAP was positively correlated. Therefore, the combination of HPV E6/E7 mRNA and YAP detection can improve the diagnostic rate for cervical lesions and predict the progression of precancerous lesions.