灰树花液态深层发酵条件研究

以菌丝体生物量和多糖含量为评价指标，通过单因素试验研究了灰树花的液态深层发酵条件，并考察不同发酵罐类型对灰 树花生长的影响，在此基础上利用50 L发酵罐进行了扩大培养。 结果表明，利用7 L搅拌式发酵罐，灰树花的最优液体深层发酵条件为培 养温度23 ℃，搅拌速度120 r/min，通气量3 L/min。 在此优化条件下，菌丝体生物量和发酵液中多糖含量分别为11.659 g/L和3.658 g/L， 且高于气升式发酵罐。 在50 L搅拌式发酵罐中成功实现了扩大培养，其菌丝体生物量和多糖含量分别为13.238 g/L和3.178 g/L。

Optimization of submerged fermentation conditions of Grifola frondosa

Using mycelia biomass and polysaccharide content as evaluation indexes, the liquid submerged fermentation conditions of Grifola frondosa were researched by single factor experiments, and the effects of different fermenter types on the growth of G. frondosa were investigated. On this basis, the amplification culture was carried out in 50 L fermentor. The results showed that in 7 L stirring fermenter, the optimum submerged fermentation conditions of G. frondosa were culture temperature 23 ℃, stirring speed 120 r/min, and ventilatory capacity 3 L/min. Under these optimum conditions, the mycelium biomass and polysaccharide content in the fermentation broth were 11.659 g/L and 3.658 g/L, respectively, which were higher than those of the air-lift fermenter. Finally, the amplification culture was successfully achieved in 50 L stirring fermenter, in which the mycelium biomass and polysaccharide content reached 13.238 g/L and 3.178 g/L, respectively.