胃泌素、环氧合酶2在不同胃黏膜病变中的表达及意义

目的 研究不同胃黏膜病变组织中胃泌素、环氧合酶2(COX-2)和Ki-67的表达,探讨胃泌素和COX-2在胃黏膜癌变过程中的作用及其相互关系.方法 96例不同胃黏膜病变组织来自胃镜活检标本.采用免疫组化染色EnVision法检测胃泌素、COX-2和Ki-67表达.结果 胃泌素在慢性浅表性胃炎(CSG)、慢性萎缩性胃炎(CAG)、肠上皮化生(IM)、异型增生(Dy)和胃癌(GC)中表达的阳性率分别为58.8%、30.0%、57.9%、68.2%和77.8%,CAG组与GC组比较,差异有统计学意义(P<0.05).COX-2在CSG、CAG、IM、Dy和GC中表达的阳性率分别为41.2%、45.0%、68.4%、72.7%和88.9%;在GC中的表达显著高于CSG(P<0.05).从CSG→GC,Ki-67增殖指数(PI)呈逐渐递增趋势;从CAD→GC,胃泌素和COX-2的表达与PI呈正相关(P<0.05).在胃癌前期病变中,胃泌素与COX-2表达有显著相关性(γ=0.4108,P<0.01),二者共同表达时,PI显著升高.结论 CAG中胃泌素王低表达,从CAG→GC,胃泌素表达逐渐升高.在胃黏膜癌变过程中,COX-2表达呈逐渐递增趋势.在胃癌前病变阶段,胃泌素和COX-2协同促进细胞增殖,胃泌素可能参与上调COX-2的表达.

Expression of gastrin and cyclooxygenase2 in different gastric mucosal lesions

Objective To investigate the expression of gastrin, cyclooxygenase-2(COX-2) and Ki-67 in dif-ferent gastric mucosal lesions and to assess the role of gastrin and COX-2 in the sequence of gastric carcinogenesis and its relationship. Methods 96 samples with different gastric mucosal lesions were obtained by gastroscope. The expression of gastrin,COX-2 and Ki-67 was examined with immunohistochemical staining technique(EnVision). Re-sults Positive rates of gastrin expression in chronic superficial gastritis (CSG), chronic atrophic gastritis (CAG), intestinal metaplasia(IM),dysplasia(Dy) and gastric cancer(GC) were 58.8 % ,30. 0% ,57.9 % ,68.2% and 77.8% ,respectively, and there were significant differences between GC and CAG(P<0.05). Positive rates of COX-2 expression in CSG,CAG,IM,Dy and GC were 41.2 % ,45.0% ,68.4 % ,72.7% and 88.9% respectively, and there were significant differences between GC and CAG(P<0.05). Ki-67 proliferation index (PI) from CSG, CAG, IM, Dy to GC also had an increasing tendency. From CAG to GC,the expression of gastrin and COX-2 was positively correlated with PI (P<0.05). The expression between gastrin and COX-2 was correlated significantly in gastric pre-cancerous lesions (P<0.01). PI was increased significantly in group with co-expression of gastrin and COX-2. Con-clusion Gastrin expression is decreased in CAG. From CAG to GC, gastrin expression is increased gradually. COX-2 expression has an increasing tendency in the sequence of gastric cancerization. From CSG to GC, PI is gradually in-creased. Gastrin and COX-2 co-promote proliferation of gastric epithelial cells,and gastrin may take part in up-regu-lation of COX-2 expression in gastric precancerous.