|
|||||
|
|
| 羊草种群遗传分化的RAPD分析I：扩增片段频率的变化 | |
| 引用本文： | 崔继哲,祖元刚,关晓铎.羊草种群遗传分化的RAPD分析I：扩增片段频率的变化[J].植物研究,2001,21(2):272-277. |
| 作者姓名： | 崔继哲 祖元刚 关晓铎 |
| 作者单位： | 1. 东北林业大学,；哈尔滨师范大学生物系, 2. 东北林业大学, 3. 辽宁省经济林研究所 |
| 基金项目： | 国家教委跨世纪优秀人才计划基金项目资助 |
| 摘 要： | 松嫩草原上分布着灰绿型和黄绿型两种叶色类型的羊草，为进一步明确这两种叶色类型种群分化的分子基础 ，对其9个种群105个个体进行了RAPD分析。15个随机引物（10nt)共扩增出149个多态性片段，总的多态位点为96．1％，黄绿型与灰绿型种群间在10．1％的多态性片段上明显不同，相似生境或同一地域的种群在一些位点上表现出相似或相同的变化；9个种群都检测到了特异性扩增。 |
| 关 键 词： | 羊草 遗传分化 RAPD 扩增片断频率 |
| 修稿时间： | 2000年12月10 |
GENETIC DIFFERENTIATION IN LEYMUS CHINENSIS POPULATIONS REVEALED BY RAPD MARKERS I: VARIATION ON THE FREQUENCIES OF AMPLIFIED FRAGMENTS |
|
| CUI Ji zhe , ZU yuan gang GUAN Xiao duo.GENETIC DIFFERENTIATION IN LEYMUS CHINENSIS POPULATIONS REVEALED BY RAPD MARKERS I: VARIATION ON THE FREQUENCIES OF AMPLIFIED FRAGMENTS[J].Bulletin of Botanical Research,2001,21(2):272-277. | |
| Authors: | CUI Ji zhe ZU yuan gang GUAN Xiao duo |
| Affiliation: | CUI Ji zhe 1,2 ZU yuan gang 1 GUAN Xiao duo 3 |
| Abstract: | Leymus chinensis is a rhizome grass species,which has 2 leaf-colored types, a grayish green and a yellowish green, in the Songnen Plain of China. To reveal the molecular bases of genetic differentiation between the 2 types, RAPD was conducted on 105 individuals from 9 populations by using 15 primers. RAPD produced 155 amplified fragments, and total percentage of polymorphic loci is 96.1. 10.1% polymorphic loci were different between the 2 types. There were similarities of certain polymorphic loci among the populations sampled in the similar habitat. There were characterized polymorphic loci for 9 populations respectively. |
| Keywords: | Leymus chinensis genetic differentiation RAPD |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
