| 黄芪多糖的提取及对体外培养成骨细胞成骨能力的影响 |
| |
| 引用本文： | 王拥军,宋莉君,孙爱贞. 黄芪多糖的提取及对体外培养成骨细胞成骨能力的影响[J]. 中国中医骨伤科杂志, 1999, 7(6): 1-4 |
| |
| 作者姓名： | 王拥军 宋莉君 孙爱贞 |
| |
| 作者单位： | 上海中医药大学!上海200032 |
| |
| 基金项目： | 上海市卫生局科研基金项目!编号：96198 |
| |
| 摘 要： | 目的：了解黄芪多糖对体外培养成骨细胞增殖、分化能力的影响。方法：采用水煮醇沉法提取黄芪多糖，用ＴＬＣ进行定性，用比色法定量，用含高低两种剂量（０．５ｍ ｇ／ｍ ｌ，５．０ｍ ｇ／ｍ ｌ）的黄芪多糖的培养液体外培养成骨细胞，ＭＴＴ法及ＡＬＰ比活性测定观察成骨细胞增殖率及细胞活性变化。结果：低浓度黄芪多糖（０．５ｍ ｇ／ｍ ｌ）及高浓度黄芪多糖（５．０ｍ ｇ／ｍ ｌ）短期时（２ 天）促进成骨细胞增殖；高浓度（５．０ｍ ｇ／ｍ ｌ）长期（４ 天）抑制成骨细胞增殖，降低其活性。结论：黄芪多糖对成骨细胞增殖、活性有双向调节作用
|
| 关 键 词： | 黄芪多糖 提取 成骨细胞 成骨能力 体外实验 |
The Refine of APS and its Effect on the Osteogenic Capacity of Osteoblasts in Vitro |
| |
| Wang Yongjun,Song Lijun,Shun Aizheng,et al$$$$Shanghai. University of Traditional Chinese Medicine. The Refine of APS and its Effect on the Osteogenic Capacity of Osteoblasts in Vitro[J]. Chinese Journal of Traditional Medical Traumatology & Orthopeics, 1999, 7(6): 1-4 |
| |
| Authors: | Wang Yongjun Song Lijun Shun Aizheng et al$$$$Shanghai. University of Traditional Chinese Medicine |
| |
| Abstract: | Objective:To understand the effect of APS on the osteogenic capacity of osteoblasts in vitro.Methods:APS was refine with water and alcoholysis,qualitatively analysis with TLC and quantitatively analysis with colorimetric analysis. Osteoblast were cultured in vitro with APS of 0.5mg/ml and 5.0mg/ml. MTT and ALP actives were used to observed the effect of APS on osteoblasts proliferation rate and osteogenic capacity.Results:APS concentration of 0.5mg/ml and 5.0mg/ml can improve the proliferation rate and osteogenic capacity through short cultured (2day) and 5.0mg/ml can decrease these effect through long cultured (4day) of normal osteoblasts.Conclusion:APS has two-way regular capacity on the osteogenic capacity of osteoblasts in vitro. |
| |
| Keywords: | Osteoblast APS Refine Osteogenic capacity In vitro |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
