不同毒力差异基因型的新生隐球菌MLST分型及交配型鉴定研究

目的：了解及比较两组毒力差异明显的新生隐球菌格鲁比变种的多位点序列分型（MLST）的特点并进行交配型鉴定。方法采用多位点序列分型（MLST）的方法，设计7个看家基因（CAP59，GPD1，LAC1，PLB1，SOD1，URA5和IGS1）的引物，扩增并分析来源分别为环境和临床的各10株新生隐球菌格鲁比变种的基因型，并鉴定实验菌株交配型，与多位点微卫星分型（MLMT）结果对比，比较不同基因分型方法在分类中的稳定性和可靠性。结果在微卫星分型中为MLMT-36的10株环境分离株，MLST分型为ST-15，而在微卫星分型中为MLMT-13型的10株临床分离株，MLST分型为ST-32，所有菌株交配型均为MAT-α。结论MLST分型结果与MLMT分型结果高度一致，提示以上两种分子分型技术在真菌分类鉴定研究中可显示对于其分离背景及进化来源的高分辨率及稳定性。

MLST genotyping and mating type identification of C. neoformans in genotypes with virulent difference

Objective To study the Multilocus Sequence Typing（ MLST）characteristics of C. neoformans var grubii and to i-dentify their mating types Methods Primers of 7 housekeeping genes（CAP59,GPD1,LAC1,PLB1,SOD1,URA5 and IGS1）were designed for PCR amplification;the genotypes of 20 environment and clinical isolates were analyzed with MLST result and the mat-ting types of these strains were identified And both the results of MLST and Multilocus Microsatellite Typing（ MLMT）were com-pared to each other for evaluating their stability and reliability in taxonomy Results The multilocus microsatellite genotype of all the 10 environment isolates was MLMT-13 in previous experiment and the MLST type of there strains was ST-15 in this experiment Sim-ilarly,all the MLMT-36 clinical isolates belong to ST-32 And all the strains were MAT-α Conclusion The MLST results are high-ly consistent with MLMT results in previous experiment which reveals that both molecular typing methods are high resoluted and sta-ble on classification of different isolation background on species or varieties level.