SHH、Gli-1、MMP-2在胃癌组织中的表达及临床意义

目的：探讨胃癌组织中刺猬信号通路（Hedgehog signaling pathway，Hh）中的音猬因子（Sonic hedgehog，Shh）和胶质瘤相关癌基因同源物-1（Glioma-associated oncogene homolog -1，Gli-1）与金属基质蛋白酶-2（Matrix metalloproteinase-2，MMP-2）的表达和临床意义。方法采用免疫组织化学方法检测40例人胃癌组织、人胃息肉组织和40例正常胃黏膜组织中音猬因子、胶质瘤相关癌基因同源物-1、金属基质蛋白酶-2蛋白的表达。结果胃癌组织中音猬因子、胶质瘤相关癌基因同源物-1、金属基质蛋白酶-2的阳性表达率分别为62.5％、67.5％、72.5％，高于胃息肉组织（阳性表达率分别为27.5％、37.5％、32.5％）和正常胃黏膜组织（阳性表达率分别为22.5％、17.5％、12.5％）（P＜0.05）；以上三者的表达与患者性别、年龄、组织学类型无关（P＞0.05）；而与分化程度、浸润深度、淋巴结转移相关（P＜0.05）；音猬因子、胶质瘤相关癌基因同源物-1、金属基质蛋白酶-2表达呈正相关。结论刺猬信号通路可能通过某些机制可上调金属基质蛋白酶-2的表达，从而增强胃癌的侵袭性。联合检测胃癌组织中音猬因子、胶质瘤相关癌基因同源物-1、金属基质蛋白酶-2的表达水平在一定程度上可以作为胃癌预后的客观参考指标。

Expression and clinical significance of Shh,Gli-1 and MMP-2 in gastric cancer

Objective To investigate the expression and clinical significance of Sonic hedgehog (Shh),Glioma-asso-ciated oncogene homolog-1 (Gli-1)and Matrix metalloproteinase-2 (MMP-2)in gastric cancer.Shh and Gli-1 are the molecules of Hedgehog (Hh)signaling pathway. MMP-2 is the member of matrix metalloproteinase family. Methods The expression of Shh,Gli-1 and MMP-2 proteins was examined by immunohistochemistry in the human gastric cancer tissues and the human gas-tric polyp and the normal gastric mucosa tissues of 40 cases. Results The positive expression rates of Shh,Gli-1 and MMP-2 in gastric cancer were 62.5%,67.5% and 72.5% respectively,which were significantly higher than those in the gastric polyp tissues (the positive expression rates were 27.5%,37.5% and 32.5% respectively)and normal gastric mucosa tissues (the positive expression rates were 22.5%,17.5% and 12.5% respectively),P<0.05.The expression of Shh,Gli-1 and MMP-2 was not correlated with the sex,age or histological type (P>0.05 ),but was correlated with depth of invasion,differentiation level and lymphonode metastasis in gastric cancer (P<0.05 ). The expression of Shh and Gli-1 was positive correlated with MMP-2. Conclusion Hedgehog (Hh) signaling pathway may have great effects on enhancing the invasive ability of gastric cancer by upregulating MMP-2 protein through some unknown mechanisms.The combined detection of the expression level of Shh,Gli-1 and MMP-2 in gastric cancer tissues might be used as an objective references for assessing the prognosis of gastric cancer.