| 高效液相色谱法测定南果梨中绿原酸的含量 |
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| 引用本文： | 辛广,刘长江,侯冬岩,吴俊青.高效液相色谱法测定南果梨中绿原酸的含量[J].食品科学,2004,25(4):150-152. |
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| 作者姓名： | 辛广 刘长江 侯冬岩 吴俊青 |
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| 作者单位： | 鞍山师范学院化学系，沈阳农业大学食品学院 |
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| 基金项目： | 辽宁省教育厅高等学校科学研究资助项目(2024201055) |
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| 摘 要： | 目的建立测定南果梨中绿原酸含量的高效液相色谱法。方法C18色谱柱(250×4.6mm,5μm);流动相甲醇-0.4%磷酸溶液(2575);检测波长327nm。结果精密度试验RSD为1.9%,n=6;加样回收率98.5%～102.6%,RSD为1.6%～2.1%。结论南果梨果心、果肉、果皮中绿原酸的含量分别为1.785、0.271、0.714mg/g。
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| 关 键 词： | 高效液相色谱法 南果梨 绿原酸 |
| 文章编号： | 1002-6630(2004)04-0150-03 |
Determination of Chlorogenic Acid of Nanguoli Pear by HPLC |
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| XIN Guang,LIU Chang-jiang,HOU Dong-yan,WU Jun-qing.Determination of Chlorogenic Acid of Nanguoli Pear by HPLC[J].Food Science,2004,25(4):150-152. |
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| Authors: | XIN Guang LIU Chang-jiang HOU Dong-yan WU Jun-qing |
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| Affiliation: | 1.Department of Chemistry,Anshan Normal University;2.College of Food Science, Shenyang Agricultural |
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| Abstract: | Objective: To establish a new HPLC method for determination of chlorogenic acid(CGA)in Nanguoli pear.Method:CGA was analysed by using C18 column(250mm×4.6mm,10μm) with detector at 327nm, and the mobile phase consistingof CH3OH-0.4% H3PO4 (25:75).Results: The recoveries of CGA was 98.5%～102.6%,precision (RSD) was 1.6%～2.1% (n=5).Conclusion:Quantitative analysis showed that the content of CGA in the core peel and pulp of Nanguoli pearwere 1.785, 0.271 and 0.714mg/g respectively. |
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| Keywords: | HPLC Nanguoli pear chlorogenic acid |
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