人参皂甙Rg3对淋巴管内皮细胞生成的影响

目的探讨人参皂甙Rg320(R)-Ginsenoside Rg3]对淋巴管内皮细胞迁移、增殖能力及凋亡的影响。方法用含不同浓度人参皂苷Rg3的培养液培养Hela细胞,收集培养上清并制备条件培养液(CM);取健康猪的胸导管内皮细胞进行分离、培养;Ⅷ因子、VEGFR-3抗体联合对淋巴管内皮细胞进行鉴定;通过划线刮除法和MTT法观察人参皂甙Rg3对淋巴管内皮细胞的迁移和增殖能力的影响;Hoechst33258荧光染色检测淋巴管内皮细胞凋亡。结果第Ⅷ因子和VEGFR-3抗体对培养的淋巴管细胞进行联合鉴定,为典型淋巴管内皮细胞;应用划线刮除法和MTT法对对照组与实验组进行比较显示:不同浓度的人参皂甙Rg3能够明显抑制淋巴管内皮细胞的增殖和游走,差异有统计学意义(P<0.01);Hoechst33258证实,经人参皂甙Rg3条件培养液处理后淋巴管内皮细胞,可观察到其核周围有凋亡小体。结论人参皂甙Rg3对淋巴管内皮细胞的迁移和增殖能力有明显的抑制作用,并且有剂量依赖性;人参皂甙Rg3能够诱导淋巴管细胞凋亡。

Influence of 20(R)-ginsenoside Rg3 on lymphangenesis

Objective To explore the effect of 20(R)-Ginsenoside Rg3 on lymphatic endothelial cell migration,proliferation and apoptosis.Methods Hela cells were cultured in media containing Rg3,and conditioned media(CM) were prepared with the culture media from hela cells;LECs which ducted from healthy pig were cultured;Ⅷ and VEGFR-3 were used to idenifty LCEs.The methods of craped line and MTT were used to examine the effectiveness of 20(R)-Ginsenoside Rg3 on lymphatic endothelial cell migration and proliferation;Hoechst33258 fluorescence staining was used to detect endothelial cell apoptosis.Results Ⅷ factor and VEGFR-3 antibody were used to identify the cultured lymphatic cells.The result showed that they were typical lymphatic endothelial cells;The scraped line and MTT method were used to compare the difference between the control group and the experimental group.The result showed:different concentrations of 20(R)-Ginsenoside Rg3 can inhibit lymphatic endothelial cell proliferation and migration,the difference was significant(P<0.01);Hoechst33258 staining confirmed that apoptotic bodies could be observed around its nuclear in 20(R)-Ginsenoside Rg3 treated LECs.Conclusion 20(R)Ginsenoside Rg3 could evidently inhibit the migration and proliferation of lymphatic endothelial cells and the effects were dosedependent;20(R)-Ginsenoside Rg3 could induce the apoptosis of LECs.