基于膜显色结果判读的基因芯片快速检测丙型肝炎基因型方法的研究

目的 研制一种新型基于膜显色的芯片，用于丙型肝炎病毒(HCV)及其基因分型的快速检测。方法 根据HCV 5’端非编码区(5’NCR)设计特异型探针和Bl物，制作DNA芯片。实验组为60份HCV RNA阳性血清，对照组为20份健康人血清。获得目的片段后与芯片杂交，通过尼龙膜上的显色信息判读基因型。同时以测序法进行双盲HCV基因分型对照检测。结果 实验组HCV芯片检测结果均为阳性，对照组均为阴性。实验组基因分型检测结果与测序结果两者符合率为96.7％。结论 用DNA芯片来检测HCV基因分型具有快速，高特异性和高灵敏度的特性，可以应用于HCV及其基因分型临床检测。

Rapid detection of the genotyping of hepatitis C virus using DNA chip with coloration methods

Objective To develop a new DNA chip with coloration, which can be used for rapid and economical detection of the genotyping of hepatitis C virus(HCV). Methods Probes and primers were designed according to the sequence of HCV 5'non-coding region(5'NCR) to fabricate DNA chip. Experimental group consisted of 60 positive serum samples and control group consisted of 20 negative serum samples. To obtain the aimed gene, then they were hybridized with DNA chip. Finally, the results showed in a nylon film. The results of DNA sequencing of samples were used as the control in double blind experimental. Results Using DNA chip, HCV was detected in positive of all serum specimens of experimental group and negative in control group. The determination of HCV genotype by DNA chip showed corresponding rate of 96.7% with those by sequence assay. Conclusion It showed higher specialty and sensitivity using DNA chip to detect the genotype of HCV. It would be valuable for the clinical genotyping of HCV.