高同型半胱氨酸血症对小鼠金属硫蛋白合成的影响

目的:观察高同型半胱氨酸(Hcy)血症时小鼠组织金属硫蛋白(MT)含量的变化。方法:小鼠腹腔注射Hcy复制高Hcy血症模型。观察肝、心和肾组织的MT和丙二醛(MDA)水平。结果:肝、心和肾组织在Hcy组MT水平较对照组分别高210%(P＜0.01)、133%((P＜0.05)和60%(P＜0.01)。而丙二醛水平则分别高6%、52%(P＜0.05)和69%(P＜0.05)。注射Hcy同时用超氧化物歧化酶(SOD)处理的动物,肝、心和肾组织MT含量较Hcy组分别低22%(P＜0.05)、33%(P＜0.05)和13%(P＞0.05)。丙二醛含量则分别降低24%(P＜0.05)、21%(P＜0.05)和48%(P＜0.01)。组织MT水平与MDA含量呈显著正相关。结论:Hcy可能通过氧化应激诱导MT生成。

Homocysteine induces metallothionein formation in mice

AIM: To observe the changes of metallothionein (MT) in various tissues of mice during hyperhomocysteinemia. METHODS: Intraperitoneal injection of homocysteine into mice induced hyperhomocysteinemia. The contents of tissue MT and malondialdehyde (MDA) in liver, heart and kidney were determined. RESULTS: Compared with control group, tissue MT levels in Hcy-group animals were increased by 210% ( P <0.01) for liver, by 133% ( P <0.05) for heart and by 60% ( P <0.01) for kidney. Tissue MDA contents were increased by 6%, 52% ( P <0.05) and 69% ( P <0.05), respectively. However, in treated animals with Hcy plus superoxide dismutase (SOD), the tissue MT contents in liver, heart and kidney were decreased by 22% ( P <0.05),33% ( P <0.05) and 13% ( P >0.05),respectively, compared with Hcy alone group. Tissue MDA contents were decreased by 24% ( P <0.05), 21% ( P <0.05) and 48% ( P <0.01). There was a positive correlation between tissue MT content and MDA level. CONCLUSION: Hcy could induce tissue MT formation through oxidative stress.