| 水苏无性系技术建立的研究 |
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| 引用本文： | 勾娇娇,宋家宝,刘松洋,徐娜,姜长阳.水苏无性系技术建立的研究[J].河南科学,2011,29(5):542-545. |
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| 作者姓名： | 勾娇娇 宋家宝 刘松洋 徐娜 姜长阳 |
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| 作者单位： | 辽宁师范大学生命科学学院,辽宁,大连,116029 |
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| 基金项目： | 辽宁省高等教育教学改革资助项目，辽宁师范大学教学改革资助项目 |
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| 摘 要： | 以水苏根茎为材料,进行了愈伤组织的诱导和分化、试管苗的生根、移栽和定植的研究,建立起水苏无性系技术.结果证明:MS+BA 0.5 mg/L+2,4-D 1.0～2.0 mg/L是愈伤组织诱导培养的理想培养基;MS+BA 0.5mg/L+2,4-D1.5mm/L是愈伤组织继代培养的理想培养基;MS+BA 0.5 mg/L...
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| 关 键 词： | 水苏 愈伤组织 无性系 |
The Study on Establishment of Clone for Stachys Japonic |
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| Gou Jiaojiao,Song Jiabao,Liu Songyang,Xu Na,Jiang Changyang.The Study on Establishment of Clone for Stachys Japonic[J].Henan Science,2011,29(5):542-545. |
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| Authors: | Gou Jiaojiao Song Jiabao Liu Songyang Xu Na Jiang Changyang |
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| Affiliation: | (Biology Scientific College,Liaoning Normal University,Dalian 116029,Liaoning China) |
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| Abstract: | In order to protect wild resources and realize artificial cultivation,the rhizome of Stachys japonic were used as material to do the research on callus induction and differentiation,rooting and transplanting of tube seed-lings,finally establish the clone of Stachys japonic.The results show that the optimum medium for callus induction,subculture and differentiation is MS+BA 0.5 mg /L+2,4-D 1.0~2.0 mg /L,MS+BA 0.5 mg /L+2,4-D 1.5 mg /L and MS+BA 0.5 mg /L+NAA 0.5 mg /L respectively.White+NAA 0.1 mg /L+IAA 0.4 mg /L is the optimum medium for adventitious buds and tube seedlings rooting and subculture.Colonization of plantlets is maintained for all biological traits which wild plant has and grows vigorously. |
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| Keywords: | stachys japonic callus clone |
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