| 丹参酮类化合物对SPC-A-1细胞的生长抑制及其构效关系探讨 |
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| 引用本文： | 石华月,张青,李慧,储婷,金辉,毛声俊.丹参酮类化合物对SPC-A-1细胞的生长抑制及其构效关系探讨[J].中国肺癌杂志,2011,14(1):7-12. |
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| 作者姓名： | 石华月 张青 李慧 储婷 金辉 毛声俊 |
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| 作者单位： | 1. 四川大学华西药学院,成都,610041
2. 四川省医学科学院,四川省人民医院,成都,610072 |
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| 基金项目： | 教育部博士点基金新教师项目(No.20070610050)资助~~ |
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| 摘 要： | 背景与目的大量研究表明丹参酮类化合物具有体外抗肿瘤作用,但很少有人综合研究丹参酮类化合物对同一种肿瘤细胞的作用情况。本文旨在比较4种丹参酮类化合物对SPC-A-1细胞的增殖抑制作用,并探讨其结构与细胞毒性之间的关联性。方法采用改良MTT法测定不同浓度的丹参酮类化合物与细胞共培养预定时间(24h、48h和72h)后对SPC-A-1细胞的增殖抑制作用;倒置显微镜下观察不同药物处理对于SPC-A-1细胞的形态学影响。结果丹参酮类化合物均能有效抑制SPC-A-1细胞增殖,其抑制作用呈明显的时间和剂量依赖性。二氢丹参酮I、丹参酮I、丹参酮IIA、隐丹参酮作用24h的IC50值分别为2.77μg/mL、6.01μg/mL、>10μg/mL和>10μg/mL;作用48h的IC50值分别为1.80μg/mL、4.04μg/mL、8.12μg/mL、8.71μg/mL;作用72h的IC50值分别为1.36μg/mL、1.69μg/mL、3.81μg/mL、7.35μg/mL。结论 4种丹参酮类化合物均对SPC-A-1细胞具有明显的增殖抑制作用,作用强度大小依次为二氢丹参酮I、丹参酮I、丹参酮IIA、隐丹参酮,提示A环为芳环时可增强细胞毒性,C环的呋喃环结构可能影响其细胞毒性,其具体作用机理尚有待探讨。
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| 关 键 词： | 丹参酮类化合物 肺肿瘤 构效关系 |
Growth Inhibition of Tanshinones on SPC-A-1 Cell Line and their Structure-activity Relationship |
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| Huayue SHI,Qing ZHANG,Hui LI,Ting CHU,Hui JIN,Shengjun MAO.Growth Inhibition of Tanshinones on SPC-A-1 Cell Line and their Structure-activity Relationship[J].Chinese Journal of Lung Cancer,2011,14(1):7-12. |
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| Authors: | Huayue SHI Qing ZHANG Hui LI Ting CHU Hui JIN Shengjun MAO |
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| Affiliation: | Huayue SHI1,Qing ZHANG1,Hui LI2,Ting CHU1,Hui JIN1,Shengjun MAO1 1West China School of Pharmacy,Sichuan University,Chengdu 610041,China,2Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital,Chengdu 610072 |
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| Abstract: | Background and objective Numerous studies have shown that Tanshinones have anti-tumor effects in vitro,but few studies were focusing on the anti-tumor activity of Tanshinones against one special cancer cell line.The aim of this study is to investigate the growth inhibition effect of four Tanshinones on SPC-A-1 cell line and the relationship between their structures and cytotoxicity.Methods The modified MTT assay was adopted to measure the inhibition effect of Tanshinones on SPC-A-1 cells at different concen... |
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| Keywords: | Tanshinone Lung neoplasms Structure-activity relationship |
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