人参皂苷Rb1对H9N2流感病毒诱导急性肺损伤小鼠抗氧化酶的影响

目的探讨人参皂苷Rb1(G-Rb1)对肺损伤小鼠抗氧化酶活力的影响。方法将195只6~8周龄BALB/c小鼠随机分为对照组、肺损伤模型组(ALI组)、人参皂苷Rb1组(G-Rb1组),每组65只。ALI与G-Rb1组采用100μL SI A/swine/HeBei/012/2008/猪流感病毒(H9N2 SIV)经鼻腔接种建立急性肺损伤模型,同时G-Rb1组腹腔注射人参皂苷Rb1液0.1 mL,剂量为10 mg/(kg·bw),连续7d;对照组鼻腔接种相同剂量生理盐水稀释的正常鸡胚尿囊液。观察临床症状、肺病理组织学变化,计算小鼠肺湿干重比、肺系数,检测小鼠肺组织T-SOD、MPO、CAT、GSH-PX活力。结果从第2天末开始ALI组大部分小鼠出现高度的精神沉郁,呼吸极度困难,采食量明显减少,体重下降。肺部明显水肿、淤血和出血,炎性细胞渗出,对照组小鼠各器官未见异常。肺系数及肺干湿重比逐渐升高,第8天开始下降,第14天趋于正常。G-Rb1组症状明显轻于攻毒组,症状出现较缓,症状较轻,死亡时间延迟,死亡率降低。在第4、6、8天,与对照组比G-Rb1组和ALI组T-SOD及CAT活力显著降低(P0.01),组间比,G-Rb1组明显高于ALI组(P0.05);在各时间点,与对照组比,ALI组GSH-PX活力显著降低(P0.01),而GRb1组则显著升高(P0.01),实验组组间差异显著(P0.01)。结论 G-Rb1在一定浓度范围内,具有提高小鼠抗氧化酶活力作用,一定程度上改善H9N2猪流感病毒对肺组织的氧化损伤。

Effect of ginsenoside Rb1 on antioxidant enzyme in mouse acute lung injury induced by H9N2 swine influenza virus

Objective To Study the effect of ginsenoside Rb1 on antioxidant enzyme in mouse acute lung injury induced by A/swine/HeBei/012/2008/swine influenza virus（H9N2 SIV）.Methods One hundred and ninety-five 6- to 8-week-old BALB/c mice were randomly divided into three groups, 65 in each.The mice in the control group were inoculated intranasally with an equivalent dilution of noninfectious allantoic fluid, and that of both the acute lung injury group（ALI group）and ginsenoside Rb1 group（G-Rb1 group）were inoculated intranasally with H9N2 SIV diluted in sterile saline, and in addition, the mice of the G-Rb1 group were treated with ginsenoside Rb1 10 mg/（kg·bw） by intraperitoneal injection continuously for seven days.Clinical signs and body weight loss were observed in eight infected mice of each group.At the same time, at the indicated time points after infection, histopathology of the lung was observed and the activities of T-SOD, MPO, CAT and GSH-PX were detected in the mouse lungs.Results After the first 2 days of infection, the mice of the ALI group showed depression, ruffled fur, reduced feed intake and weight loss. Furthermore, pulmonary edema, hemorrhage, and a number of inflammatory cells exuding from the pulmonary alveoli were observed in the lungs of infected mice. Lung coefficient and lung wet/dry weight ratio was increased gradually. The changes began to decline on the 8th day and tend to be normal on the 14th day. The organs of mice of the control group showed no abnormality.For mice in the G-Rb1 group, clinical symptoms were significantly improved, survival time was prolonged, and mortality was decreased. On the 4th, 6th and 8th day after infection：the activity of both T-SOD and CAT was significantly reduced（P〈0.01）in the mice of ALI and G-Rb1 groups compared with that of the control group, but the index of G-Rb1 group was significantly higher than that of the ALI group（P〈0.05）.At each time point after infection, the GSH-PX activity was significantly lower（P〈0.01）in the ALI group compared with that of the control group, but the GSH-PX activity of G-Rb1 group was significantly higher than that of the ALI group（P〈0.01）, with a significant difference between the two groups（P〈0.01）.Conclusions G-Rb1 can improve the activity of antioxidase in mouse acute lung injury induced by H9N2 SIV, and to some extent, G-Rb1 can ameliorate the acute lung injury induced by H9N2 SIV infection.