OPCRP填料的SBMBBR处理低温污水脱氮细菌多样性试验

针对低温污水生物脱氮效率低问题，采用有机高分子复合硬性颗粒（OPCRP）-SBMBBR反应器处理低温污水，与传统SBR反应器对比，通过Miseq高通量测序技术分析了2套反应器中活性污泥的细菌菌群多样性及组成结构丰度差异，揭示高效处理低温污水优势脱氮菌群。结果表明:在水温（6.5±1）℃条件下，OPCRP-SBMBBR反应器出水脱氮效果及污泥沉降速率均明显提高；投加填料有助于提高活性污泥系统内硝化反硝化菌多样性和相对丰度，即优势氨氧化菌（AOB）、亚硝酸盐氧化菌（NOB）、厌氧反硝化菌总相对丰度分别由SBR （R1）的3.9%、3.47%、15.87%增加到OPCRP-SBMBBR （R2）的5.21%、5.26%、23.64%。异养硝化-好氧反硝化菌种红环菌科、Enterobacteriaceae、Terrimonas，分别由R1的2.77%、1.63%、2.43%增加到R2的3.3%、3.11%、2.59%；R2独有的好氧反硝化菌种包括假单胞菌属、氢噬胞菌属等，其相对丰度分别为1.17%、0.79%。R1、R2中优势好氧反硝化菌种总相对丰度分别为10.66%、17.35%，优势硝化菌种总相对丰度分别为7.37%、10.47%，优势硝化反硝化菌种总相对丰度分别为28.65%、43.32%，为低温污水中生物脱氮提供了良好的细菌环境。

DIVERSITY OF NITROGEN REMOVAL BACTERIA IN LOW TEMPERATURE SEWAGE TREATED BY A SBMBBR WITH OPCRP FILLER

In view of the low biological denitrification efficiency of low-temperature sewage, the low-temperature sewage was treated by an organic polymer composite hard particle bio-carrier(OPCRP)-SBMBBR reactor. Compared with the traditional SBR reactor, two sets of reactors were analyzed by Miseq high-throughput sequencing technology. The difference in bacterial flora diversity and compositional abundance of activated sludge in the medium revealed the efficient treatment of the dominant nitrogen-depleting bacteria in low-temperature sewage. The results showed that under the condition of water temperature at (6.5±1) ℃, the effluent denitrification effect and sludge settling rates of OPCRP-SBMBBR reactor were obviously improved. Adding fillers could improve the diversity of nitrification and denitrifying bacteria in activated sludge system. And the relative abundance, the total abundance of dominant ammonia oxidizing bacteria (AOB), nitrite oxidizing bacteria (NOB) and anaerobic denitrifying bacteria increased from 3.9%, 3.47%, and 15.87% of SBR (R1) to 5.21%, 5.26%, 23.64% of (OPCRP)-SBMBBR (R2), respectively. Heterotrophic nitrification-aerobic denitrifying bacteria, including Rhodobacteraceae, Enterobacteriaceae, and Terrimonas increased from 2.77%, 1.63%, and 2.43% of R1 to 3.3%, 3.11%, and 2.5% of R2; and unique aerobic reaction nitrifying strains of R2, Pseudomonas, Hydrogenobacter, their relative abundances were 1.17% and 0.79%, respectively. The total relative abundance of dominant aerobic denitrifying strains in R1 and R2 were 10.66% and 17.35%, respectively; the total relative abundance of dominant nitrifying strains in R1 and R2 was 7.37% and 10.47%, respectively; the total relative abundance of dominant nitrifying and denitrifying strains in R1 and R2 was 28.65% and 43.32%, respectively, which provided a good bacterial environment for biological nitrogen removal in low temperature sewage.