| 多拷贝rol基因对转基因杨树生长及内源激素的影响 |
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| 引用本文： | 郝宇,梁海永,杨敏生.多拷贝rol基因对转基因杨树生长及内源激素的影响[J].林业科学,2010,46(5). |
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| 作者姓名： | 郝宇 梁海永 杨敏生 |
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| 作者单位： | 1. 河北农业大学园林与旅游学院,保定071000;天津国土资源和房屋职业学院艺术设计系,天津300270
2. 河北农业大学林学院,保定,071000 |
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| 基金项目： | “十一五”国家高技术研究发展计划(863计划)项目(2009AA10Z107);;教育部博士点基金资助项目(20070086004) |
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| 摘 要： | 对转入多拷贝rolB、rolC基因的三倍体毛白杨进行高生长量、生根率和内源激素(IAA、ABA)含量等指标的测定,以研究多拷贝rol基因在转基因植物中的表达。结果显示:转Ri质粒三倍体毛白杨再次分别转入rolB、rolC基因后,部分株系试管苗的生长受到了抑制,但各株系生根率均有不同程度提高;rolB基因转化植株的内源IAA平均含量高于rolC基因转化植株,其IAA/ABA值亦高于rolC基因转化植株。试验结果表明rol基因的多拷贝促使转化植株快速大量生成毛状根。
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| 关 键 词： | rol基因 三倍体毛白杨 生长 内源激素 |
Effect of the Multi-Copied Gene on the Growth and the Endogenous Hormones of the Transgenic Poplar |
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| Hao Yu,Liang Haiyong,Yang Minsheng.Effect of the Multi-Copied Gene on the Growth and the Endogenous Hormones of the Transgenic Poplar[J].Scientia Silvae Sinicae,2010,46(5). |
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| Authors: | Hao Yu Liang Haiyong Yang Minsheng |
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| Affiliation: | 1. College of Landscape and Travel/a>;Agricultural University of Hebei Baoding 071000/a>;2. College of Forestry/a>;3. The Art Department of Design/a>;Tianjin Land Resources and House Vocational College Tianjin 300270 |
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| Abstract: | In order to study the expression of multiple rol genes,height-growth,rate of rooting,endogenous hormone (IAA,ABA) and other parameters of the triploid Populus tomentosa were measured. The results indicated that growth of some transgenic plants was inhibited while the rooting rates of all the transgenic plants were increased to various degrees. Compared with the transgenic plants with rolC gene,the plants transferred with rolB gene were higher in both the IAA content and the rates of IAA /ABA on average. The... |
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| Keywords: | rol gene triploid Populus tomentosa growth endogenous hormone |
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