| 海洋假单胞菌SB12产表面活性剂的研究 |
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| 引用本文： | 杨利娟,李卫平,邱立,黄通旺,胡忠.海洋假单胞菌SB12产表面活性剂的研究[J].汕头大学学报(自然科学版),2008,23(3):38-44. |
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| 作者姓名： | 杨利娟 李卫平 邱立 黄通旺 胡忠 |
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| 作者单位： | 汕头大学生物系,广东,汕头,515063 |
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| 基金项目： | 国家自然科学基金，霍英东教育基金，广东省科技厅科技计划 |
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| 摘 要： | 从汕头湾海底沉积物中分离到24株表面活性剂产生菌,对其中一株产生物表面活性剂能力最强的菌株(SB12)进行鉴定、发酵条件优化及生物表面活性剂特性的初步研究.经鉴定,确定该菌株为假单胞菌(Pseudomonas sp.).对其产生物表面活性剂的发酵条件进行优化,确定了最佳单因子发酵条件:氮源为蛋白胨,盐浓度为0.1%,pH 9.5,温度为25℃,培养时间为6 d;优化后的最小表面张力为19.77 mN/m.分析发酵液中生物表面活性剂的特性发现:菌株SB12产生的生物表面活性剂具有良好的乳化性能,乳化力达91%;产生的生物表面活性剂具有较广的温度和pH适应范围.
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| 关 键 词： | 生物表面活性剂 菌株筛选 培养条件优化 假单胞菌 |
Research on the Producing of SB12 from Marine |
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| YANG Li-juan,LI Wei-ping,QIU Li,HUANG Tong-wang,HU Zhong.Research on the Producing of SB12 from Marine[J].Journal of Shantou University(Natural Science Edition),2008,23(3):38-44. |
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| Authors: | YANG Li-juan LI Wei-ping QIU Li HUANG Tong-wang HU Zhong |
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| Affiliation: | (Department of Biology., Shantou University, Shantou 515063, Guangdong, China) |
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| Abstract: | Twenty-four biosurfactant-producing microorganisms were isolated from the bay of Shantou and Strain SB12 was the best one for produced biosurfactant. Strain SB12 was identified as Pseudomonas sp.. After cultural condition optimization, the optimal fermentation conditions of SB12 for producing biosurfactant were： peptone was as nitrogen source, 0.1% NaC1, pH9.5, cultivated at 25 ℃ for 6 d. The surface tension reduced to 19.77 mN/m. The biosuffactant produced by SB12 had good emulsify efficiency（its emulsification index was 91%）, good rang of temperature adaptation（even bear the high temperature up to 121 ℃） and also a wide adaptable pH range of 4. 0-12.0. |
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| Keywords: | biosurfactant strain screening cultural condition optimization Pseu-domonas sp |
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