| 软枣猕猴桃多聚半乳糖醛酸酶提取条件的优化 |
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| 引用本文： | 刘月,李书倩,张博,刘长江,辛广.软枣猕猴桃多聚半乳糖醛酸酶提取条件的优化[J].食品科学,2012,33(10):115-118. |
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| 作者姓名： | 刘月 李书倩 张博 刘长江 辛广 |
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| 作者单位： | 1.沈阳农业大学食品学院
2.鞍山师范学院化学系 |
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| 基金项目： | 国家公益性行业(农业)科研专项(200903013) |
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| 摘 要： | 以软枣猕猴桃为实验材料，确定多聚半乳糖醛酸酶最优提取条件和最适活性分析条件。在提取过程中，以酶比活力为指标确定缓冲液的最适pH值、离子浓度、DTT添加量对PG提取效果的影响，通过正交试验确定最佳提取条件。结果表明：最优提取条件为pH5.5、0.05mol/L 乙酸缓冲液为提取液，加入0.1mol/L NaCl、1mmol/L DTT；最适活性分析条件为反应温度40℃、反应时间90min。
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| 关 键 词： | 软枣猕猴桃 多聚半乳糖醛酸酶 提取 活性分析 |
Optimization of Extraction Conditions for Polygalacturonase from Actinidia arguta |
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| LIU Yue,LI Shu-qian,ZHANG Bo,LIU Chang-jiang,XIN Guang.Optimization of Extraction Conditions for Polygalacturonase from Actinidia arguta[J].Food Science,2012,33(10):115-118. |
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| Authors: | LIU Yue LI Shu-qian ZHANG Bo LIU Chang-jiang XIN Guang |
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| Affiliation: | (1. College of Food Science, Shenyang Agricultural University, Shenyang 110866, China；
2. Department of Chemistry, Anshan Normal University, Anshan 114007, China) |
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| Abstract: | Actinidia arguta was used as the experimental material to explore the optimal extraction conditions and enzymatic activity analysis conditions of polygalacturonase(PG).The optimal extraction process parameters were explored by orthogonal tests through evaluating the effects of pH,ion concentration and DTT addition amount on extraction rate PG.The results indicated that the optimal extraction process condtions for PG were extraction pH of 5.5,acetate buffer concentration of 0.05 mol/L,NaCl concentration of 0.1 mol/L and DTT concentration of 1 mmol/L.The optimal enzymatic activity analysis conditions were reaction temperature of 40 ℃ and reaction time of 90 min. |
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| Keywords: | Actinidia arguta polygalacturonase(PG) extraction enzymatic activity analysis |
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