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不同因素对淮山茎尖愈伤组织诱导的影响
引用本文:赵艳红,何海旺,韦本辉,甘秀芹,蒋菁.不同因素对淮山茎尖愈伤组织诱导的影响[J].安徽农业科学,2007,35(30):9475-9475,9481.
作者姓名:赵艳红  何海旺  韦本辉  甘秀芹  蒋菁
作者单位:广西农业科学院经济作物研究所,广西南宁,530007;广西农业科学院经济作物研究所,广西南宁,530007;广西农业科学院经济作物研究所,广西南宁,530007;广西农业科学院经济作物研究所,广西南宁,530007;广西农业科学院经济作物研究所,广西南宁,530007
基金项目:广西农业科学院科技发展基金项目(2005011);广西创新能力建设项目(桂科能0443002-6);广西农业科学院重点优势学科项目(2004006Z).
摘    要:以3个淮山品种为材料,比较不同培养基及培养条件对淮山茎尖愈伤组织诱导的影响。结果表明,MS+6-BA 2.0 mg/L+NAA 2.0mg/L+蔗糖30.0 g/L+琼脂4.0 g/L是诱导淮山茎尖愈伤组织较好的培养基,诱导率达88.9%;光照与黑暗两种不同的培养条件对愈伤组织的诱导无显著差异。

关 键 词:淮山  诱导  愈伤组织  培养条件
文章编号:0517-6611(2007)30-09475-01
修稿时间:2007-05-30

Effect of the Different Factors on the Induction of Callus in Yam Stem-tip Cutlure
ZHAO Yan-hong et al.Effect of the Different Factors on the Induction of Callus in Yam Stem-tip Cutlure[J].Journal of Anhui Agricultural Sciences,2007,35(30):9475-9475,9481.
Authors:ZHAO Yan-hong
Affiliation:Cash Crop Research Institute;Guangxi Academy of Agricultural Sciences;Nanning;Guangxi 530007
Abstract:The effect of the different factors on induction of callus in yam stem-tip culture was compared,and three yam varieties were cultured with the different culture media and conditions.The result showed that,the culture medium of MS 6-BA 2.0 mg/L NAA 2.0 mg/L sucrose 30.0 g/L agar 4.0 g/L was best than others,and its rate of induction was 88.9%,at the same time,there was no significant difference in the induction of callus when yam was cultured in the two conditions of light and dark.
Keywords:Yam  Induction  Callus  Culture condition
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