专性菌系对石油烃污染土壤的修复性能

从焦化废水厂的活性污泥中采用“邻苯二酚-石油烃”双底物驯化获得石油烃的专性降解菌系，在最佳环境条件下研究其对石油污染土壤的修复性能.高通量测序分析显示，驯化后的专性菌系以产黄杆菌属（Rhodanobacter sp.）为主，占比41%.最适降解温度为30~35℃，最佳pH值为7~8.聚山梨酯-80（吐温80）可作为碳源促进专性菌系的生长，提高石油烃的降解效率，并且添加吐温80比十二烷基硫酸钠（SDS）更利于石油烃的降解.研究表明，当吐温80的浓度为5CMC（即375mg/L）时，石油烃的降解效率最高.在最佳环境条件下，为期80d修复试验，构建的专性菌系石油烃降解效率稳定在77%，这证实了双底物驯化模式获得的专性菌系对石油污染土壤具有良好的修复性能.

Remediation performance of constructed specific bacteria on petroleum hydrocarbon contaminated soil

The specific mixed bacteria were domesticated from the activated sludge of coking plant using double substrate domestication. The remediation capacity of the specific bacteria on petroleum hydrocarbon contaminated soil was studied under the optimum environmental conditions. High-throughput sequencing analysis revealed that Rhodanobacter sp. was the dominant bacterium, which accounted for 41%. The optimum degradation temperature and pH were 30~35℃ and 7~8, respectively. Tween 80can be utilized as carbon source to promote the growth of the specific bacteria and improve the degradation efficiency of petroleum hydrocarbons. Moreover, Tween 80showed greater promotion on petroleum hydrocarbon degradation in contaminated soil as compared to SDS. The greatest degradation efficiency of petroleum hydrocarbons occurred at Tween 80concentration of 5CMC (i.e. 375mg/L). Furthermore, the degradation efficiency of petroleum hydrocarbons after 80days reached 77% under the optimum environmental conditions, which evidenced the outstanding remediation performance of the specific bacteria on petroleum hydrocarbon contaminated soil.