| 大孔树脂吸附法纯化紫薯花色苷的研究 |
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| 引用本文： | 魏好程,米 聪,王瑞芳,何传波,吴国宏,熊何健.大孔树脂吸附法纯化紫薯花色苷的研究[J].食品安全质量检测技术,2015,6(8):3186-3191. |
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| 作者姓名： | 魏好程 米 聪 王瑞芳 何传波 吴国宏 熊何健 |
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| 作者单位： | 集美大学食品与生物工程学院, 福建省食品微生物与酶工程重点实验室,集美大学食品与生物工程学院,集美大学食品与生物工程学院,集美大学食品与生物工程学院,集美大学食品与生物工程学院,集美大学食品与生物工程学院 |
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| 基金项目： | 福建省自然科学基金(2013J01134)、福建省教育厅(面上)项目(JA13177)、福建省食品微生物与酶工程重点实验室开放(M20130910)、厦门市科技计划项目(3502Z20153014)。 |
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| 摘 要： | 目的研究大孔吸附树脂吸附法纯化紫薯花色苷成分。方法采用大孔吸附树脂静态和动态吸附解吸实验,结合花色苷p H示差法检测技术,分别考察了D101、AB-8、XDA-7、HPD-722、HPD-750、HPD-450、XDA-6、NKA-II、NKA9和S-8 10种吸附树脂对紫薯花色苷的吸附和洗脱性能,探讨大孔树脂柱层析纯化工艺。结果 XDA-7大孔吸附树脂对紫薯花色苷的吸附和洗脱性能较好。吸附过程中上样液浓度为450 mg/L,样液p H为4.0,上样速率为1 BV/h,树脂的饱和吸附容量为10.2 mg/g;洗脱液为60%乙醇溶液,洗脱速率为2BV/h时,洗脱解析率在94%以上,纯化效果较好。结论 XDA-7大孔吸附树脂可用于紫薯花色苷的纯化应用,该纯化分离工艺简单快速,适合紫薯类花色苷的纯化制备。
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| 关 键 词： | 紫薯 花色苷 纯化 |
| 收稿时间： | 2015/6/30 0:00:00 |
| 修稿时间： | 2015/8/19 0:00:00 |
Purification of anthocyanins from purple sweet potato by macroporous ad-sorption resin |
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| WEI Hao-Cheng,MI Cong,WANG Rui-Fang,HE Chuan-Bo,WU Guo-Hong and XIONG He-Jian.Purification of anthocyanins from purple sweet potato by macroporous ad-sorption resin[J].Food Safety and Quality Detection Technology,2015,6(8):3186-3191. |
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| Authors: | WEI Hao-Cheng MI Cong WANG Rui-Fang HE Chuan-Bo WU Guo-Hong and XIONG He-Jian |
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| Affiliation: | College of Food and Biological Engineering, Jimei University, Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering,College of Food and Biological Engineering, Jimei University,College of Food and Biological Engineering, Jimei University,College of Food and Biological Engineering, Jimei University,College of Food and Biological Engineering, Jimei University and College of Food and Biological Engineering, Jimei University |
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| Abstract: | Objective To study the purification of anthocyanins from purple sweet potato by macroporous adsorption resins. Methods The static & dynamic adsorption and desorption characteristics of macroporous adsorption resins including D101, AB-8, XDA-7, HPD-722, HPD-750, HPD-450, XDA-6, NKA-II, NKA9 and S-8 for anthocyanins were investigated, combined with pH differential detection technology, and the purple potato anthocyanins macroporous adsorption purification technology and parameters optimization were discussed. Results Resin XDA-7 showed a satisfactory adsorption-desorption capacity for purification of anthocyanins from purple sweet potato. In the course of adsorption, the concentration of adsorption solution was 450 mg/L with the adsorption solution pH 4.0, the flowing rate was 1 BV/h, and the saturated adsorption capacity of the resin was 10.2 mg/g. In the course of elution, the elution rate was more than 94% when the eluent was 60% ethanol aqueous and its flowing rate was 2 BV/h. Conclusion The developed method is easy to implement and can be applied to the purification of anthocyanins from purple sweet potato. |
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| Keywords: | purple sweet potato anthocyanins purification |
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