子宫颈鳞状细胞癌中P53、MDM2的表达与HPV16感染的关系

目的探讨子宫颈鳞状细胞癌中P53、MDM2蛋白的表达及其与高危HPV16型感染的关系。方法免疫组化二步法检测43例子宫颈鳞状细胞癌、20例CIN、15例慢性子宫颈炎中P53、MDM2的表达,HPV16的检测应用原位杂交法。结果43例子宫颈鳞状细胞癌中HPV、P53、MDM2的表达率分别为46.51%、34.88%、25.58%,CIN中的表达率分别为45%、5%、15%,HPV16在慢性宫颈炎中的表达率为6.67%,P53、MDM2未见阳性表达,三组比较有统计学意义（P〈0.05）。P53、MDM2在HPV16阳性和阴性两组间的表达差异无统计学意义。结论HPV16与子宫颈鳞状细胞癌的发生密切相关,P53、MDM2的表达并不因HPV16的存在而消失。

Expression of P53 and MDM2 in cervical squamous cell carcinoma and its correlation with HPV16 infection

Objective To investigate the expression of P53 and MDM2 in cervical squamous cell carcinoma and explore their correlation with HPV16 infection. Methods Immunohistochemistry was used to detect P53 and MDM2 proteins in 43 cases of cervical squamous cell carcinoma, 20 cases with CIN and 15 cases with chronic cervicitis. In situ hybridization was used to detect HPV16. Results The positive rates of HPV16,P53,MDM2 in 43 cases of cervical squamous cell carcinoma were 46.51%,34.88%,25.58% respectively. The positive rates of HPV16,P53,MDM2 in CIN were 45% ,5% ,15% respectively. Positive rate of HPV16 in chronic eervicitis was 6.67%, P53 and MDM2 were not detected in chronic cervicitis. The differences among there groups were significant（P〈0.05）. Similar average expression levels of P53,MDM2 were found in the presense or absence of HPV16, without significant differences between the two groups. Conclusion HPV 16 infection is related to cervical squamous cell carcinoma. Expression of P53 and MDM2 were not abrogated by the presence of HPV16.