针刺对高血脂合并脑缺血大鼠海马区Caspase-9蛋白表达及神经行为学的影响

目的:探讨针刺对脑缺血损伤的神经保护机制及高脂血症与脑缺血损伤的关系。方法:雄性SD大鼠70只,随机分为正常对照组、高血脂模型组、脑缺血模型组、高血脂合并脑缺血模型组、脑缺血治疗组、高血脂合并脑缺血治疗Ⅰ组、高血脂合并脑缺血治疗Ⅱ组,每组10只。采用经典高脂配方饲料喂养并结合化学刺激诱导血栓性闭塞大脑中动脉造成大鼠高血脂合并局灶性脑缺血联合模型。脑缺血治疗组针刺"百会""水沟"穴;高血脂合并脑缺血治疗Ⅰ组先电针"三阴交""丰隆",每日1次,治疗10d后再造脑缺血模型;高血脂合并脑缺血治疗Ⅱ组脑缺血后针刺"三阴交""丰隆""百会""水沟"穴,每日1次,治疗7d。治疗结束后,用神经行为学症状评分标准评定大鼠的神经症状,应用免疫组化方法观察大鼠海马区Caspase-9蛋白表达的变化。结果:大鼠局灶性脑缺血后Caspase-9表达增加(P<0.01),以高血脂合并脑缺血模型大鼠尤为明显(P<0.01);针刺后Caspase-9表达明显减少,其中高血脂合并脑缺血治疗Ⅰ组较高血脂合并脑缺血治疗Ⅱ组减少更显著(P<0.01)。同时各治疗组大鼠神经行为学评分亦明显降低。结论:针刺可改善高血脂合并脑缺血大鼠的神经行为学症状,减轻Caspase-9的过度表达,表明针刺治疗高脂血症可有效地预防和减轻脑缺血损伤程度。

Effect of Electroacupuncture on Caspase-9 Expression in Hippocampus and Neuroethology in Hyperlipemia Rats with Concurrent Cerebral Ischemia

Objective To observe the effect of electroacupuncture(EA)on hippocampal apoptosis protein caspase-9 expression and neuroethology in hyperlipemia plus cerebral ischemia(HL-CI)rats.Methods Seventy male SD rats were randomized into control,hyperlipemia(HL),CI,HL-CI,CI+EA,HL-CI+EAⅠ,and HL-CI+EA Ⅱgroups,with 10 cases in each.HL model was established by feeding the animals with high fat forage for 6 weeks and CI model was established by occlusion of the unilateral middle cerebral artery.EA(1-3 mA,15 Hz)was applied to bilateral "Sanyinjiao"(SP 6)and "Fenglong"(ST 40)for 20 min every time;and "Baihui"(GV 20)and "Shuigou"(GV 26)were punctured and stimulated by twirling the acupuncture needle with hand continuously for 1 min.Acupuncture was given once daily for 17 days(beginning from the 10th day on before CI)in HL-CI+EA Ⅰ group,and for 7 days(beginning after CI)in HL-CI+EA Ⅱ group.The expression of hippocampal Caspase-9 was detected with immunohistochemistry.The score of neuroethology was also measured according to modified Bederson's method.Results In comparison with normal control and hyperlipemia model groups,Caspase-9 immune reaction(IR)positive cells in the hippocampus in HL-CI group increased significantly(P<0.01).After acupuncture Caspase-9 IR-positive cells decreased remarkably.In comparison with group HL-CI+EA Ⅱ,Caspase-9 IR-positive cells decreased significantly in HL-CI+EA Ⅰ(P<0.01).The score of neuroethology also degraded obviously.Conclusion Acupuncture can improve neuroethology symptom,lessen over expression of hippocampal Caspase-9,and prevent CI injury in hyperlipemia rats with concurrent CI.