| 烟草花叶病毒山西番茄分离物的全基因组序列测定及分析 |
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| 引用本文： | 庞小静,赵慧琪,王德富,刘勇,牛颜冰.烟草花叶病毒山西番茄分离物的全基因组序列测定及分析[J].中国蔬菜,2017,1(6):50-55. |
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| 作者姓名： | 庞小静 赵慧琪 王德富 刘勇 牛颜冰 |
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| 作者单位： | 1. 山西农业大学生命科学学院，山西太谷 030801；;2. 湖南省农业科学院植物保护研究所，湖南长沙 410125 |
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| 基金项目： | 公益性行业（农业）科研专项（201303028），国家自然科学
基金项目（31540050） |
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| 摘 要： | 为明确山西省晋中市侵染番茄的病毒种类,采用双链RNA(double-stranded RNA,ds RNA)技术和非序列依赖PCR扩增(sequence-independent amplification,SIA)方法对感病番茄进行分子鉴定。序列测定及分析发现,具有花叶、卷曲症状的番茄为烟草花叶病毒(Tobacco mosaic virus,TMV)所侵染。进一步克隆TMV番茄分离物(TMV-SXFQ)的全基因组序列,得到TMV-SXFQ(Gen Bank登录号为JX993906)全长为6 394 bp,含4个开放阅读框(open reading frame,ORF)。序列比对分析表明,TMV-SXFQ与烟草花叶病毒属其他分离物的同源性为86.3%~99.6%;系统进化分析表明,TMV-SXFQ与韩国分离物IM聚为一簇、亲缘关系最近。
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| 关 键 词： | 番茄 病原鉴定 烟草花叶病毒 序列分析 |
Complete Whole Genome Sequence Measure and Analysis of Tobacco mosaic virus Infecting Tomato in Shanxi |
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| PANG Xiao-jing,ZHAO Hui-qi,WANG De-fu,LIU Yong,NIU Yan-bing.Complete Whole Genome Sequence Measure and Analysis of Tobacco mosaic virus Infecting Tomato in Shanxi[J].China Vegetables,2017,1(6):50-55. |
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| Authors: | PANG Xiao-jing ZHAO Hui-qi WANG De-fu LIU Yong NIU Yan-bing |
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| Affiliation: | 1.College of Life Sciences，Shanxi Agricultural University，Taigu 030801，Shanxi，China；;2.Plant Protection Institute，;
Hunan Academy of Agricultural Science，Changsha 410125，Hunan，China |
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| Abstract: | To identify the virus pathogen inducing tomato（Solanum lycopersicum L.）mosaic and curl
disease at Jinzhong City of Shanxi Province，the technology of double-stranded RNA（dsRNA）and sequenceindependent
amplification（SIA）were used．Results of SIA and sequencing showed that there was Tobacco
mosaic virus（TMV）in affected tomato leaves．Further cloning the whole genome sequence of TMV（TMVSXFQ），
the TMV-SXFQ was gained（GenBank No. JX993906），which was 6 394 bp in length and presented a
typical Tobamovirus genome organization with 4 ORFs．Sequence comparative analysis showed that the homology
of TMV-SXFQ and Tobacco mosaic virus and other isolates was 86.3%-99.6%．Phylogenetic analysis suggested
that TMV-SXFQ isolates had closeer ties consanguinity with IM strain from South Korea and these 2 isolates formed
an independent branch． |
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| Keywords: | Tomato Pathogen identification Tobacco mosaic virus（TMV） Sequence analysis |
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