鸡源致病性大肠杆菌的分离鉴定及药敏试验

为了解鸡源致病性大肠杆菌的生物学特性和耐药性,试验通过采集病料、细菌分离培养、16S rRNA基因鉴定、致病性试验、药敏试验等方法。结果表明:分离出的20株细菌的培养特征、镜检特征均符合大肠杆菌特征;16S rRNA基因序列经BLAST比对与大肠杆菌同源性达99.9%～100%,确定20株分离菌为鸡源大肠杆菌;20株大肠杆菌的致病力为40%～100%;10株鸡源致病性大肠杆菌对15种抗菌药物均呈现多重耐药,耐药率为33.3%～93.3%,耐药情况较严重和复杂。

A Study on Isolation Identification and Drug Sensitivity Test of Chicken-derived Pathogenic Escherichia Coli

To understand the biological traits and drug resistance of pathogenic Escherichia coli from chicken, we conducted the study by collecting disease materials, bacterial isolation and culture, 16S rRNA gene identification, pathogenicity test, drug sensitivity test and other tests. The results show that the culture and microscopic traits of the 20 isolates are consistent with the traits of E. coli; the 16S rRNA gene sequence was 99.9%-100% homologous to E. coli by BLAST, and 20 isolates were identified as chicken-derived Escherichia coli; 20 strains of Escherichia coli have a pathogenicity of 40% to 100%; 10 strains of chicken-derived pathogenic Escherichia coli have multi-drug resistance to 15 antimicrobial agents, and the resistance rate is 33.3%-93.3% . The drug-resistant situation is serious and complicated