鸡蛋黄防龋抗体的制备、抗体效价及持久性观察

目的：从鸡蛋中提取特异性抗体IgY，并研究鸡蛋黄抗体免疫应答的持久性，以探索一种新的被动免疫防龋方法。方法：以变形链球菌GTF高表达株S.mutans B29-33及茸毛链球菌S.sobrinus 6715免疫母鸡。采用硫酸铵沉淀法提取鸡蛋黄抗体，SDS－PAGE鉴定抗体性质，分光光度法检测蛋白质含量，间接酶联免疫吸附法（ELISA）测定鸡血清及鸡蛋黄抗体效价滴度变化。结果：SDS－PAGE鉴定证实：用硫酸铵沉淀法可提取出鸡蛋黄抗体，其性质为IgG，蛋白质含量约为每毫升7.00mg鸡蛋黄。免疫应答持续时间超过24周。结论：鸡蛋黄抗体来源充足，抗体含量高，并能在一段较长时间内从免疫母鸡鸡蛋黄中获取，有较好的应用前景。

Antibody of immunoglobulin Y isolated from egg yolk of hens immunized wi th S.mutans B29-33 and S.sobrinus 6715

AIM: The specificity of egg yolk antibody IgY against cariogenic bacteria and the protracted time of the antibody were detected to find a new passive immunization method. METHODS:50 hens were divided into two groups and injected with S.mutans B29-33 and S.sobrinus 6715 separately. The egg yolk antibody IgY purified by Sodium sulfate was determined with SDS-PAGE. The protein content was evaluated by absorption spectrometry method. Indirect ELISA was applied to evaluate the specific activity of IgY. RESULTS:SDS-PAGE showed that the purified egg yolk was IgG. The protein content was 7.00mg/ml. The specific activity lasted more than twenty-four weeks. CONCLUSION:Egg yolk antibody can be purified conveniently with high protein content. It has high specificity against cariogenic bacteria and can be obtained in a long time. So it can be used as a suitable source of antibody for passive immunization.