襄阳大头菜腌制液膜醭细菌群落结构研究

使用Miseq高通量测序技术对3个襄阳大头菜腌制液膜醭样品的细菌微生物群落结构进行了解析。在门水平上，变形菌门（Proteobacteria）和硬壁菌门（Firmicutes）平均含量分别为62.12%和34.13%。在属水平上，相对含量＞1.0%的细菌属分别为盐单胞菌（Halomonas）、色盐杆菌属（Chromohalobacter）、海杆菌属（Marinobacter）、盐厌氧菌属（Halanaerobium）、四联球菌（Tetragenococcus）和碱杆菌属（Alkalibacillus），其相对含量为26.31%、16.49%、8.13%、24.87%、6.13%和1.16%。在操作分类单元水平（OTU）上，发现14个平均相对含量＞1.0%的核心OTU，其中OTU4083（隶属于盐厌氧菌属）、OTU4400（隶属于色盐杆菌属）和OTU846（隶属于盐单胞菌属）的累计平均相对含量为39.50%。由此可见，襄阳大头菜膜醭中的细菌微生物主要由隶属于变形菌门和硬壁菌门的6个属构成，且膜醭共有大量的核心细菌菌群。

Diversity of bacterial microflora of biomembrane in Xiangyang mustard root brine

Three biomembrane samples of Xiangyang mustard root brine were collected and the bacterial microbiota community structure was studied by Miseq high throughput sequencing technology. Results showed that at phyla level, Proteobacteria and Firmicutes were the most dominant, with the relative abundance of 62.12% and 34.13%, respectively. At Genus level, the genus with relative content more than 1.0% were Halomonas, Chromohalobacter, Marinobacter, Halanaerobium, Tetragenococcus, and Alkalibacillus, with the relative abundance of 26.31%, 16.49%, 8.13%, 24.87%, 6.13%, and 1.16%, respectively. At the operational taxonomic unit (OTU) level, results showed that 14 OTUs were with the relative abundance of more than 1.0%, and the cumulative average relative contents of OTU4083 (member of Halanaerobium), OTU4400 (member of Chromohalobacter), and OTU846 (member of Halomonas) was 39.50%. The results indicated that the bacterial microbiota of biomembrane in Xiangyang mustard root brine was mainly consisted by 6 genera, including Proteobacteria and Firmicutes, and biomembrane samples shared a large number of core bacterial microbiome.