高效液相色谱法测定饮料中的新红、诱惑红及赤藓红

利用高效液相色谱法测定饮料中的新红、诱惑红及赤藓红含量，确定了检测条件为：Agilent C18 (150 mm×4.6 mm，5 μm)色谱柱，柱温25 ℃，流动相为乙酸铵-甲醇(92∶8, V/V)，流速1.0 mL/min，进样量10 μL，检测波长254 nm。结果表明，新红、诱惑红及赤藓红在0～50 μg/mL范围内线性关系良好(R2=0.999 8)，平均加样回收率为91.0%～96.8%，平均相对标准偏差(RSD)为0.4%～2.9%，新红、诱惑红及赤藓红的检出限分别为0.10 mg/kg、1.00 mg/kg及0.70 mg/kg。该方法快速准确，适用于饮料中的新红、诱惑红及赤藓红含量的测定。

Determination of new red,fancy red and erythrosine in drinks by HPLC

The contents of new red, fancy red and erythrosine in drinks were determined by high performance liquid chromatography. The determination conditions were determined as chromatographic column Agilent C18(150 mm×4.6 mm, 5 μm) with ammonium acetate-water (92∶8, V/V) as mobile phase, column temperature 25 ℃, flowing rate 1.0 ml/min, injection volume 10 μL, detection wavelength 254 nm. The results showed that new red, fancy red and erythrosine showed good linear relationship in the range of 0-50 μg/ml (R2=0.999 8), the average recovery rate was 91.0%-96.8%, the relative standard deviation (RSD) was 0.4%-2.9%, and the detection limit of new red, fancy red and erythrosine was 0.10 mg/kg, 1.00 mg/kg and 0.70 mg/kg. The method was quick and accurate for detection of the new red, fancy red and erythrosine contents in drinks.