FMDV OA/58病毒株VP3蛋白结构的模拟与分析

以口蹄疫病毒株OA／58 RNA为模板，反转录并扩增目的cDNA，然后与pMD18-T载体连接并转化JM109菌株，提取的重组质粒用凝胶电泳、PCR和BarnHⅠ、HindⅢ双酶切法鉴定。分析表明，口蹄疫病毒VP1、VP2、VP3和VP4在核苷酸水平上的变异率是无差异的（P〉0．05）；而它们在氨基酸水平上的变异率差异显著（P〈0．05）。该毒株与20株源于GenBank中的VP3氨基酸序列比较发现其保守区主要位于第1～24、26～35、37～43、45～57、61～122、124～173、175～209、211～220位。运用同源模建，OA／58 VP3蛋白三维空间结构可分为A，B，C3个结构区域。保守区氨基酸残基在维持VP3蛋白的空间构象和功能方面具有重要作用。

Construction and Analysis of VP3 Protein from A Foot-and-mouth Disease Virus Strain OA/58

Foot-and-mouth disease virus strain OA/58 RNAs were used as templates for RTPCR.The amplified cDNA products were cloned into pMD18-T vectors and transformed into JM109.The recombinant plasmids were identified by electrophoresis,PCR,and analysis of tow cleavages with BamH I and Hind III.After analyzing the difference among VP1,VP2,VP3,VP4,at the nucleotide level,the mutation rates of these 4 encoding sequences were no difference(p>0.05),however,at the amino acid level,those mutation rates were different(p<0....