大鼠Meynert基底核注入海人藻酸后c-fos原癌基因蛋白在脑纹状体边缘区及边缘系统内的表达

为研究Meynert基底核与纹状体边缘区以及脑内其他跟学习记忆有关的边缘系统结构间的关系，将海人藻酸注射于大鼠Meynert基底核后，观察c－fos原癌基因蛋白在脑内的表达。海人藻酸注射4h后，脑内只有少量c－fos阳性表达：海人藻酸注射8h后，脑内c－fos原癌基因蛋白表达增至最高峰。在海马、齿状回、中央杏仁核、纹状体、边缘区中有大量密集的c－foc阳性表达。在终纹床核、丘脑网状核、外侧隔核、丘脑下部视上核、室旁核、前下托及嗅内皮质等处可见较多c－foc阳性表达。在伏隔核、视前区、丘脑底核、黑质外侧部等部位可见少量c－fos阳性表达。海人藻酸注射Meynert基底核16h后，除海马、齿状回、中央杏仁核、纹状体及边缘区中有较多表达外，其余部位阳性标记基本消失。但脑腹侧的梨状前皮质、嗅结节、杏仁皮质核及前嗅核等部位出现大量的c－fos阳性表达。大脑皮质也可见较多c－fos阳性表达。海人藻酸注射48h后，脑内各部位的c－fos阳性反应均消失。本研究结果提示Meynert基底核和纹状体边缘区以及跟脑内其它与学习记忆有关的边缘系统结构间有密切的功能联系。

Expression of oncogene c-fos protein in the marginal division and limbic system following kainic acid injection into the basal nucleus of Meynert in rat

To investigate the relationship betWeen marginal division ofstriatum and other structures related to learning and memoryfunction of the brain, 0. 1% kainic acid was stereotaxically injected into the Meynert's hasal nucleus, and the expression ofoncogenecabs protein in the brain was observed by using the immunohistochemistry. 4 h later, there were few cabs-positive cells in the brain f8 h later, expression of c-fos was increased at the peak. Many dark stained c-fos-positive neurons were found in marginal divisionof the striatUm, hippocampus, dentate gyms, central amygdaloid nucleus, striatum, reticular nucleus of the thalamus. the bed nucleusof the stria terminals, thalamic reticular nucleus, lateral septal nucleus. supraoptic nucleus, paraventricular nucleus of hypothalamus,presubiculum and entorhinal cortex. A few c-fos-positive reactions were found in the accumbens. preoptic are4 subthalamic nucleus.lateral part of substantia nigra. 16h later, the positive expression disappeared, except in the hippocampus, dentate gyms, centralamygdaloid nucleus, striatUm, and marginal division of striatUm. However. numerous c-fos-positive cells were observed in theprepiriform cortex, olfactory tUbercle, conical amygdaloid nucleus, anterior olfactory nucleus and cortex. 48 h later, all the c-fosreaction in the brain disappeared. These results suggest that the function connections exist betWeen the Meynert's hasal nucleus ormarginal division and limbic structUres which are concerned with learning and memory function. Therefore, it is assumed that themarginal division is related to the fijnction of learning and memory of the brain.