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| 香蕉RAPD反应体系的建立 | |
| 引用本文： | 刘金福,潘东明,代立春,庄西卿,李英豪,赖钟雄,吴少华.香蕉RAPD反应体系的建立[J].中国农学通报,2009,25(16):51-55. |
| 作者姓名： | 刘金福 潘东明 代立春 庄西卿 李英豪 赖钟雄 吴少华 |
| 作者单位： | 1. 福建农林大学园艺学院,福州,350002;福建农林大学林学院,福州,350002 2. 福建农林大学园艺学院,福州,350002;福建农林大学园艺产品贮藏保鲜研究所,福州,350002 3. 福建农林大学林学院,福州,350002 4. 福建农林大学园艺学院,福州,350002 5. 福建省热带作物科学研究所,福建漳州,363001 |
| 基金项目： | 福建省科技重大专项基金果茶专项基金，福建省科技厅重点项目 |
| 摘 要： | 利用改良的CTAB方法从香蕉叶片中提取高质量的DNA。在参考一般RAPD分析反应程序的基础上，经过反复试验，确定适合香蕉PCR扩增程序为：94℃预变性 5min；94℃变性 1min；38℃退火 1min；72℃延伸 2min；变性ó延伸，循环45次；最后72℃延伸 2min。PCR扩增的体系（总体积25μL）为：模板DNA 20ng，dNTP 200μmol/L，10×PCR Buffer2.5μL，引物0.20μmol/L，Taq酶0.75U，ddH2O17.85μL。 |
| 关 键 词： | 香蕉 模板DNA RAPD |
| 收稿时间： | 2009-02-24 |
| 修稿时间： | 2009-04-30 |
The Establishment of RAPD Reaction Program of Fujian Banana |
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| Liu Jinfu,Pan Dongming,Dai Lichun,Chen Qingxi,Zhuang Xiqing,Li Yinghao.The Establishment of RAPD Reaction Program of Fujian Banana[J].Chinese Agricultural Science Bulletin,2009,25(16):51-55. | |
| Authors: | Liu Jinfu Pan Dongming Dai Lichun Chen Qingxi Zhuang Xiqing Li Yinghao |
| Affiliation: | Liu Jinfu, Pan Dongming, Dai Lichun, Chen Qingxi, Zhuang Xiqing, Li Yinghao (1Horticulture College of Fujian Agriculture and Forestry University, Fuzhou 350002;2Forestry College of Fujian Agricglture and Forestry University, Fuzhou 350002;3Institute of Storage Science and Technology for Hortic ultural Product, Fujian Agric ulture and Forestry University, Fuzhou 350002;4Fujian Tropical Crop Scientific Institute, Zhangzhou Fujian 363001 ) |
| Abstract: | Using the improved method of CTAB, high purity DNA was obtained from banana leaves. Based on the common RAPD reaction program and adjusting experiments, the optimal amplification program was described as follows: 94 ℃ for 5 min. 45 cycles at 94 ℃ for 1 min, 38 ℃ for 1 min and 72 ℃ for 2 min; 72 ℃ for 2 min at last. PCR system(25 μ1 total volumes)contains: 20 ng of genomic DNA, 200 μmol/L of dNTP, 2.5 μl of 10×PCR Buffer, 0.20 μmol/L of primer, 0.75 U Taq DNA polymerase and 17.85 μl of ddH2O. |
| Keywords: | RAPD banana template DNA RAPD |
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