Effect of collagen II coating on mesenchymal stem cell adhesion on chitosan and on reacetylated chitosan fibrous scaffolds |
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Authors: | Guillaume R Ragetly Dominique J Griffon Hae-Beom Lee and Yong Sik Chung |
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Affiliation: | (1) Department of Veterinary Clinical Medicine, University of Illinois, Urbana, IL 61802, USA;(2) Department of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea;(3) Department of Textile Engineering, Chonbuk National University, Jeonju, Republic of Korea |
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Abstract: | The biocompatibility and biomimetic properties of chitosan make it attractive for tissue engineering but its use is limited
by its cell adhesion properties. Our objectives were to produce and characterize chitosan and reacetylated-chitosan fibrous
scaffolds coated with type II collagen and to evaluate the effect of these chemical modifications on mesenchymal stem cell
(MSC) adhesion. Chitosan and reacetylated-chitosan scaffolds obtained by a wet spinning method were coated with type II collagen.
Scaffolds were characterized prior to seeding with MSCs. The constructs were analyzed for cell binding kinetics, numbers,
distribution and viability. Cell attachment and distribution were improved on chitosan coated with type II collagen. MSCs
adhered less to reacetylated-chitosan and collagen coating did not improve MSCs attachment on those scaffolds. These findings
are promising and encourage the evaluation of the differentiation of MSCs in collagen-coated chitosan scaffolds. However,
the decreased cell adhesion on reacetylated chitosan scaffold seems difficult to overcome and will limit its use for tissue
engineering. |
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