头皮针对脑缺血模型大鼠神经生长因子的影响

目的 :研究头皮针对脑缺血模型大鼠神经生长因子 (NGF)的影响 ,并与电针组比较。方法 :线栓法制备大鼠脑缺血模型 ,脑组织甲醛固定切片 ,运用免疫组化方法检测各组神经生长因子的变化情况。结果 :术后 6hr与术后 15天组内神经功能评分比较 ,模型组无明显差异 (P >0 .0 5) ,电针组与针刺组有显著性差异 (P <0 .0 1) ;组间 15天时比较 ,电针组与针刺组无明显差异(P >0 .0 5) ,两组与模型组比较均有显著性差异 (P <0 .0 1)。在第 15天时神经生长因子免疫组化染色比较 ,电针组与模型组有明显差异 (P <0 .0 5) ,针刺组与模型组有显著性差异 (P <0 .0 1) ,针刺组与电针组有明显差异 (P <0 .0 5)。结论 :头皮针可促进神经生长因子产生并延长神经生长因子产生时限 ,这可能是头皮针减轻脑缺血损伤并促使肢体功能恢复的机理之一

Effect of Scalp Acupuncture on Cerebral Nerve Growth Factor Expression in Cerebral Ischemia Rats

Objective: To study the effect of scalp acupuncture on cerebral nerve growth factor (NGF) expression in cerebral ischemia rats. Methods: A total of 50 Wistar rats were evenly randomized into control, sham operation, model, electroacupuncture (EA) and hand acupuncture groups. Cerebral ischemia model was established by occlusion of the right middle cerebral artery with a nylon thread embolus. “Baihui"(GV 20) and “Qubin"(GB 7) were punctured and stimulated with an EA stimulator (EA group) and hand manipulation (hand acupuncture). The treatment was given once daily and continuously for 15 days. The brain tissue samples were fixed with formaldehyde, sliced up, and then stained with immunohistochemical technique for observing NGF expression. Results: Auto comparison between 6 hr and 15 d after operation in neurological defect scores of model group showed no significant difference (P>0.05), but in both EA and hand acupuncture groups, the neurological defect scores decreased apparently after treatment (P<0.01). There were notable differences between EA group and model group, and between hand acupuncture group and model group in the score (P< 0.01), but no distinct difference was found between EA group and hand acupuncture group in the score (P>0.05). It showed a favorable effect of acupuncture in improving cerebral ischemia induced neurological dysfunction. Immunohistochemical staining of the cerebral cortex tissue displayed that NGF immunoreaction (IR) positive neurons (+) appeared in only one animal of model group and no IR positive neurons were found in control and sham operation group. The mild (+), moderate () and strong () IR positive neurons in EA and hand acupuncture groups were found in 7 and 1, 2 and 7, 1 and 2 animals respectively. Rank test showed that the IR density of both EA and hand acupuncture group was stronger than that of model group (P<0.05～0.01) and the effect of hand acupuncture group was significantly superior to that of EA group (P<0.05). Conclusion: Scalp acupuncture can improve neurological function and upregulate the expression of cortical NGF in cerebral ischemia rats.