| 不同产地一点红总生物碱的提取与分析 |
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| 引用本文： | 周吴萍,陈晓伟,韦媛媛,李军生.不同产地一点红总生物碱的提取与分析[J].时珍国医国药,2009,20(12). |
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| 作者姓名： | 周吴萍 陈晓伟 韦媛媛 李军生 |
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| 作者单位： | 广西工学院,广西,柳州,545006 |
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| 摘 要： | 目的 通过测定不同产地一点红中总生物碱含量,建立一种以总生物碱含量变化为基础的一点红质量测定方法.方法 利用单因素法考察最佳提取条件,采用紫外-可见分光光度法,以苦参碱为对照品,于414 nm波长处测定总生物碱含量.结果 各产地样品的总生物碱含量在0.441%～0.756%之间,贵州贵阳的一点红总生物碱含量较高.结论 用所建立的方法检测一点红药材质量,重复性好,准确度高.
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| 关 键 词： | 一点红 总生物碱 质量测定方法 |
Extraction and Analysis of Total Alkaloids Contents in Emilia sonchifolia from Different Regions |
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| ZHOU Wu-ping,CHEN Xiao-wei,WEI Yuan-yuan,LI Jun-sheng.Extraction and Analysis of Total Alkaloids Contents in Emilia sonchifolia from Different Regions[J].Lishizhen Medicine and Materia Medica Research,2009,20(12). |
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| Authors: | ZHOU Wu-ping CHEN Xiao-wei WEI Yuan-yuan LI Jun-sheng |
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| Abstract: | Objective To establish a total alkaloids-based method for determining the quality of the Emilia sonchifolia. Methods The optimum extracting condition was set up by single-factor method, and the total alkaloids were determined by UV-visible spectrophotometry under 414 nm using matrine as a control. Results The total alkaloids contents in the samples from different regions were 0.441～0.756%. The results showed the total alkaloids content in the sample which collected from Guiyang of Guizhou province was the highest. Conclusion The method has good duplication and high exactitude for quality determination of Emilia sonchifolia. |
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| Keywords: | Emilia sonchifolia Total alkaloids Quality-determination method |
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