| L-谷氨酸发酵的变温控制工艺研究 |
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| 引用本文： | 宋翔,陈宁.L-谷氨酸发酵的变温控制工艺研究[J].天津化工,2010,24(6):27-29. |
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| 作者姓名： | 宋翔 陈宁 |
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| 作者单位： | [1]天津渤海职业技术学院,天津300402 [2]天津科技大学,天津300222 |
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| 摘 要： | 以黄色短杆菌GDK-9为供试菌株,首先考察了不同的培养温度(30~36℃)对L-谷氨酸摇瓶发酵的影响。结果发现,在34℃条件下菌体生长情况最好,且L-谷氨酸产量最高,可达86g/L。基于上述结果,研究了3种变温控制模式下L-谷氨酸摇瓶发酵过程。得出如下结论:0~6h发酵温度为34℃,6h后每隔6h提高1℃,并采用这种温度控制方式进行5L自控发酵罐试验,36h后可积累L-谷氨酸130g/L,比同类研究的产量(102 g/L)提高了27%。
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| 关 键 词： | L-谷氨酸 发酵 变温控制 |
Study on variable temperature control strategy of L-glutamic acid production |
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| SONG Xiang,CHEN Ning.Study on variable temperature control strategy of L-glutamic acid production[J].Tianjin Chemical Industry,2010,24(6):27-29. |
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| Authors: | SONG Xiang CHEN Ning |
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| Affiliation: | 1.Tianjin Bohai Vocational Technical College,Tianjin 300402; 2.Tianjin University of Science and Technology,Tianjin 300222) |
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| Abstract: | In this paper,L-glutamic acid production by Brevibacterium flavum GDK-9 at various temperature was investigated to study the effect of temperature on L-glutamic acid fermentation.Different cultivation temperatures ranging from 30℃ to 36℃ were evaluated in shake flasks.The result indicated that 34℃ was the best for cell growth and the maximum L-glutamic acid production（86 g/L）was obtained.Based on the result,three temperature-shifted controlling manners were studied.The conclusion was as follows： 0-6h,culture temperature is 34℃,and it is increased 1℃ every 6h after 6h.In 5L jar fermentor,130 g/L L-glutamic acid was accumulated in 36h using this controlling manner,which was increased 27% compared with before. |
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| Keywords: | L-glutamic acid fermentation variable temperature control |
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