薄层扫描法测定发酵前后猪胆汁中猪去氧胆酸的含量

 目的:测定猪胆汁中猪去氧胆酸的含量；比较发酵前（4℃冰箱内旋转7d）及发酵后(38℃恒温自然发酵7 d)猪胆汁中猪去氧胆酸含童的变化。方法:薄层扫描法测定了5批发酵前、后的猪胆汁。结果:发酵前猪胆汁中猪去 氧胆酸的平均含量为0.012%(RSD=3.3%,n=5)～0.017%(RSD=4.0%,n=5)，发酵后猪胆汁中猪去氧胆酸的平均含量为0.044%(RSD=3.4%,n=5)～0.059%(RSD=3.6%,n=5)。结论:发酵后猪胆汁中猪去氧胆酸的量是发哮前的3.0～3.7倍。

Determination of hyodeoxycholic acid in pig bile before and after fermentation by TLC scanning method

OBJECTIVE:Hyodeoxycholic acid in pig bile was determined and the contents of hyodeoxycholic acid in pig bile before ( put in refrigerator for 7 days at 4℃)and after fermentation ( naturally fermentated for 7 days at constant temperature 380C) were compared. METHOD: TLC scanning method was used to determine pig bile before and after fermentation in five batches. RESULTS: The average content of hyodeoxycholic acid in pig bile before fermentation was 0. 012%(RSD=3.3%，n=5)～0. 017 %(RSD=4.0%，n=5) and after fermentation 0.044%(RSD=3.4%，n=5)～0.059%(RSD=3.6%，n=5).CONCLUSION: The content of hyodeoxycholic acidl in pig bile after fermentation was 3.0～3.7 times as much as that before fermentation.