Two monoclonal antibodies against small-cell lung cancer show existence of synergism in binding |
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Authors: | Shin'ichi Saito Tamotsu Inoue Ichiro Kawase Hideki Hara Yoshiro Tanio Isao Tachibana Seiji Hayashi Masatoshi Watanabe Machiko Matsunashi Tadashi Osaki Tomiya Masuno Susumu Kishimoto |
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Affiliation: | (1) The Third Department of Internal Medicine, Osaka University School of Medicine, 1-1-50 Fukushima, 553 Fukushima-ku, Osaka, Japan;(2) Faculty of Health and Sport Science, Osaka University, 560 Toyonaka, Osaka, Japan |
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Abstract: | Summary Murine IgG1 monoclonal antibodies (mAbs), ITK-2 and ITK-3, were generated against a small-cell lung cancer (SCLC) cell line. Enzyme-linked immunosorbent assay using a variety of established cell lines as substrates, immunoperoxidase staining of freshly frozen tissue sections, and fluorescence-activated cell sorter analysis of peripheral blood leukocytes showed that these mAbs recognize a part of the SCLC-associated cluster 1 antigen. In immunoprecipitation studies, both ITK-2 and ITK-3 bound to a 145-kDa glycoprotein of SCLC cell membrane extracts, as did MOC-1 and NKH-1, which both recognize the cluster 1 antigen. However, because the binding of125I-labeled ITK-2 to SCLC cells was not inhibited by MOC-1 or NKH-1, the binding site of ITK-2 on SCLC cells appeared to be different from that of either MOC-1 or NKH-1. Unexpectedly, binding of125I-labeled ITK-2 to SCLC cells increased in the presence of ITK-3. This ITK-3-induced increase in ITK-2 binding was due partly to an increase in the number of binding sites for ITK-2 on SCLC cells. Addition of ITK-3 may, therefore, improve the effectiveness of ITK-2-based tumor detection or therapy. |
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Keywords: | Monoclonal antibodies Small-cell lung cancer Synergy of mAb binding |
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