Biological imaging of HEK293 cells expressing PLCgamma1 using surface-enhanced Raman microscopy |
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Authors: | Lee Sangyeop Kim Sungyong Choo Jaebum Shin Soon Young Lee Young Han Choi Ha Young Ha Seunghan Kang Kyungho Oh Chil Hwan |
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Affiliation: | Department of Applied Chemistry, Hanyang University, Ansan 426-791, South Korea. |
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Abstract: | Surface-enhanced Raman scattering (SERS) imaging has been used for the targeting and imaging of specific cancer markers in live cells. For this purpose, Au/Ag core-shell nanoparticles, conjugated with monoclonal antibodies, were prepared. The procedures to label live cells with those bimetallic nanoprobes have been developed and used for highly sensitive SERS imaging of live cells. In the present study, live HEK293 cells expressing PLCgamma1 have been used as the optical imaging target. Our results demonstrate the potential feasibility of SERS imaging technology for the highly sensitive imaging of cancer biomarkers in live cells. |
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