表达Harpin蛋白的芽孢杆菌工程菌防治水稻白叶枯病的研究

本文研究了生防促生芽孢杆菌OKB105、FZB42和以其为宿主菌的可外泌表达Harpin蛋白激发子的基因工程菌OKBHF、FZBHarpin对水稻白叶枯病的防治效果。菌株OKB105、FZB42、OKBHF和FZBHarpin温室防效分别为31.7%、23.6%、43.2%和56.7%;田间防效分别为29.8%、21.8%、40.4%和43.1%,表达Harpin蛋白的芽孢杆菌工程菌的防病效果显著高于出发菌株。两株生防芽孢杆菌及其工程菌对水稻均有良好的促生作用,特别是对水稻植株的生长促进明显,其中FZBHarpin使植株增高达15.86%。田间产量统计结果显示,OKBHF增产效果最显著,为14%,产量达8869 kg·hm^-2。工程菌比出发菌株显著提高水稻抗病性和促进植物生长,在农业生产上具有应用的潜力。

Biocontrol Efficacy on Rice Bacterial Leaf Blight of Genetically Engineered Bacillus Expressing Harpin

In this study,Bacillus OKB105、FZB42 and their genetically engineered bacteria OKBHF and FZBHarpin,which express and secret Harpin proteins,were determined for their biocontrol efficacy on rice bacterial leaf blight.Tests indicated that,control efficiacies of the four strains OKB105,FZB42,OKBHF and FZBHarpin were 31.7%,23.6%,43.2% and 56.7%,respectively,in greenhouse,while 29.8%,21.8%,40.4% and 43.1%,respectively,in field.Compared with patent strains,genetically engineered bacteria showed higher biocontrol efficacy and more significant plant growth-promoting capability,especially FZBHarpin which increased rice height by 15.86%.Results also indicated that yield of rice treated with OKBHF reached up to 8869 kg ·ha^- 1 by 14% increment.