组胺对白色脂肪细胞脂肪合成以及调控的机制研究

目的：在体外探索组胺对脂肪合成的影响及其作用机制。方法：培养3T3L1细胞,诱导为白色脂肪细胞,运用RT-PCR技术检测组胺和苯海拉明对白色脂肪细胞中脂肪细胞脂肪酸结合蛋白2（aP2 mRNA）水平的影响。结果：组胺可以增加分化成熟的3T3L1小鼠胚胎成纤维细胞（前脂肪细胞）aP2的表达,剂量效应图显示组胺为20μmol/L时aP2表达量为最高,时间效应图显示在共同孵育6 h后aP2表达量达到最高;苯海拉明与同剂量组胺相比,可以显著降低aP2的表达（P〈0.05）。结论：组胺可以刺激分化成熟的3T3L1细胞的aP2的表达,苯海拉明可以抑制组胺的效应。组胺能增加脂肪细胞的脂肪合成,维持白色脂肪细胞能量代谢的平衡。

Effect of histamine on fat synthesis and regulation in the white adipocytes: a mechanism research

AIM：To study the effect of histamine on fat synthesis in the differentiated 3T3L1 cell in vitro and its mechanisms. METHODS：The 3T3L1 cells cultured and induced it to white fat cells.The levels of aP2 mRNA in the adipocytes under the treatment of histamine and diphenhydramine were detected by RT-PCR techniques.RESULTS：Histamine increased the expression of aP2 mRNA in the adipocytes,the concentration-dependent effect curve discovered the highest expression of aP2 mRNA was at 20 μmol/L,the time-dependent effect curve discovered the highest expression of aP2 mRNA was at 6 h.Compare with the same concentration of histamine,diphenhydramine decreased the expression of aP2 mRNA（P0.05）.CONCLUSION：Histamine increased the expression of aP2 mRNA in the differentiated 3T3L1 cell.Diphenhydramine inhibited the expression of aP2 mRNA.Histamine can increase the fat synthesis and maintain the balance of energy metabolism in the white adipocytes.