Liver iron overload induced by tamoxifen in diabetic and non-diabetic female Wistar rats |
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Authors: | Carlos André Nunes Jatobá Adriana Augusto de Rezende Sarah Jane de Paiva Rodrigues Maria Margareth de Almeida Câmara Maria das Graças Almeida Francisco Freire-Neto Luiz Reginaldo Menezes da Rocha Aldo Cunha da Medeiros José Brandão-Neto Maria Célia de Carvalho Formiga Ítalo Medeiros de Azevedo Ana Maria de Oliveira Ramos |
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Affiliation: | (1) Postgraduate Course in Health Sciences, Federal University of Rio Grande do Norte (UFRN), Natal, RN, CEP 59010-180, Brazil;(2) Department of Clinical Pharmacy and Toxicology, UFRN, Natal, RN, CEP 59010-180, Brazil;(3) Department of Pathology, UFRN, Natal, RN, CEP 59010-180, Brazil;(4) Department of Surgery, UFRN, Natal, RN, CEP 59010-180, Brazil;(5) Department of Clinical Medicine, UFRN, Natal, RN, CEP 59010-180, Brazil;(6) Department of Statistics, UFRN, Natal, RN, CEP 59010-180, Brazil |
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Abstract: | Tamoxifen (TX), a drug used in the treatment of breast cancer, may cause hepatic changes in some patients. The consequences
of its use on the liver tissues of rats with or without diabetes mellitus (DM) have not been fully explored. The purpose of
this study was to evaluate the correlation between plasma hepatic enzyme levels and the presence of iron overload in the hepatic
tissue of female Wistar rats with or without streptozotocin-induced DM and using TX. Female rats were studied in control groups:
C-0 (non-drug users), C-V (sorbitol vehicle only) and C-TX (using TX). DM (diabetic non-drug users) and DM-TX (diabetics using
TX) were the test groups. Sixty days after induced DM, blood samples were collected for glucose, alanine aminotransferase
(ALT), aspartate aminotransferase (AST) alkaline phosphatase (ALP) and bilirubin measures. Hepatic fragments were processed
and stained with hematoxylin and eosin, Masson’s trichrome, Perls. The hepatic iron content was quantified by atomic absorption
spectrometry. AST, ALT and ALP levels were significantly elevated in the DM and DM-TX groups, with unchanged bilirubin levels.
Liver iron overload using Perls stain and atomic absorption spectrometry were observed exclusively in groups C-TX and DM-TX.
There was positive correlation between AST, ALT and ALP levels and microscopic hepatic siderosis intensity in group DM-TX.
In conclusion, TX administration is associated with liver siderosis in diabetic and non-diabetic rats. In addition, TX induced
liver iron overload with unaltered hepatic function in non-diabetic rats and may be a useful tool for investigating the biological
control of iron metabolism. |
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Keywords: | Liver Hemosiderin Siderosis Iron metabolism Diabetes mellitus |
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