葛根素对妊娠期糖尿病大鼠糖脂代谢作用的研究

目的?观察葛根素对妊娠期糖尿病大鼠糖脂代谢的影响。方法?选用高脂喂养的妊娠d1的Wistar大鼠，予链脲佐菌素（STZ）25?mg/kg一次性腹腔注射，注射72?h后，空腹血糖稳定在6.67~16.67?mmol/L为GDM模型复制成功。将造模成功的大鼠随机分为模型组、葛根素组各6只，另取6只正常妊娠大鼠为正常对照组，从妊娠d1起葛根素组予以葛根素0.25?g/(kg·d）灌胃，正常组及模型组给予等容积0.9%的生理盐水灌胃，至产出子鼠。观察各组大鼠25周时血清空腹血糖(FBG)、空腹胰岛素（FBINS）、血脂、肿瘤坏死因子-α（TNF-α）、白介素-6（IL-6）、瘦素（Leptin）、脂联素（APN）的变化情况，qPCR检测肝脏组织中瘦素受体、胰岛素受体及脂肪组织中TNF-α、IL-6、APN、Leptin mRNA的表达。结果?葛根素组大鼠FBG、甘油三酯（TG）、低密度脂蛋白（LDL-C）、总胆固醇（TC）、TNF-α、IL-6、Leptin与模型组比较均有明显下降（P<0.05~0.01），葛根素组大鼠高密度脂蛋白（LDL-H）、APN水平与模型组比较有显著上升（P<0.05~0.01），葛根素组大鼠脂肪组织中胰岛素受体mRNA的表达较模型组显著增加，葛根素组大鼠肝脏组织中TNF-α、Leptin mRNA的表达较模型组明显下降（P<0.05）。结论?葛根素可改善妊娠期糖尿病大鼠糖脂代谢，其作用机制可能与调节TNF-α、Leptin、APN水平相关。 

Effect of Puerarin on Glucose and Lipid Metabolism in Gestational Diabetic Mellitus Rats

OBJECTIVE?To observe the influence of puerarin on glucose and lipid metabolism in gestational diabetic mellitus(GDM) rats. METHODS?High-fat feeding pregnant wistar rats were given streptozocin （STZ） 25?mg/kg intraperitoneally on the first day of pregnant. After injection for 72 hours， rats with FBG between 6.67-16.67?mmol/L were gestational diabetic models， which were randomly divided into two groups: GDM model group(MG， using 0.9% NaCl)， puerain treated group(TG， using puerain 0.25?g/(kg·d)， 6 rats in each group. And we selected 6 pregnant rats as normal gestation group(NG， using 0.9% NaCl). The rats were given drugs by intragastric administration until the offspring were born. The levels of FBG， insulin， serum lipid， TNF-α， IL-6， leptin and adiponection were observed at the age of 25 weeks. Leptin receptor insulin receptor， TNF-α， IL-6， adiponection and leptin mRNA expression in fat and liver tissues were detected by qPCR. RESULTS?Compared with GDM model group， the levels of FBG， insulin， TG， TC， LDL-C， TNF-α， IL-6 and leptin of puerarin treated group were significantly decreased(P<0.05， P<0.01). The levels of HDL-C and adiponection of puerarin treated group were significantly increased(P<0.05， P<0.01). The mRNA expression of IR in TG was higher than MG， and the mRNA expression of TNF-α and leptin in TG were decreased significantly （P<0.05）. CONCLUSION?Puerarin can improve glucose and lipid metabolism in gestational diabetic rats， the mechanism may be related to the regulation of TNF-α， IL-6， leptin and adiponectin levels.